Cold Spring Harb Protoc. 2020 Jun 1;2020(6):101196. doi: 10.1101/pdb.prot101196.
This protocol differs from other transformation procedures in that the bacterial culture is grown at 18°C rather than the conventional 37°C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1 × 10 to 3 × 10 transformed colonies/µg of plasmid DNA are typical.
本方案与其他转化程序的不同之处在于，细菌培养在 18°C 下进行，而不是常规的 37°C。除此之外，该方案并无特别之处，遵循相当标准的流程。在标准实验室条件下，每微克质粒 DNA 的转化菌落数通常为 1×10 到 3×10。
