BioPhysics Group, Centre for Stem Cells and Regenerative Medicine, Institute of Healthcare Engineering and Department of Mechanical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Adv Biosyst. 2020 Aug;4(8):e2000019. doi: 10.1002/adbi.202000019. Epub 2020 Jun 2.
In this review, a brief history of this unrivaled technology, flow cytometry, is provided, highlighting its past and present advances, with particular focus on "flow cell" technologies. Flow cytometry has truly revolutionized high-throughput single cell analysis, which has tremendous implications, from laboratory to the clinic. This technology embodies what is truly referred to as cross fertile research, merging the physical with the life sciences. This review introduces the recent notable advancements in flow cell technology. This advancement sees the complete removal of liquid sheath flow, which has advanced the technology with the possibility of both the reduction in its foot print, while also simplifying the flow cells explored in cytometry. Interestingly, the novel sheathless flow cell technology demonstrated herein has the flexibility for handling both heterogeneous cell populations and whole organisms, thus demonstrating a versatile flow cell technology for both flow cytometry and fluorescent-activated cell sorting.
在这篇综述中，简要介绍了这项无与伦比的技术——流式细胞术的历史，重点介绍了其过去和现在的进展，特别关注“流室”技术。流式细胞术真正实现了高通量单细胞分析的革命，从实验室到临床都具有巨大的意义。这项技术体现了真正意义上的交叉研究，将物理与生命科学融合在一起。本文介绍了流室技术的最新显著进展。这项进展完全去除了液鞘流，从而推进了该技术的发展，有可能缩小其体积，同时简化细胞术探索的流室。有趣的是，本文展示的新型无鞘流室技术具有处理异质细胞群体和整个生物体的灵活性，因此为流式细胞术和荧光激活细胞分选提供了一种多功能的流室技术。
