College of Engineering, China Agricultural University, Beijing, China.
Anim Sci J. 2020 Jan-Dec;91(1):e13397. doi: 10.1111/asj.13397.
To investigate the yeast population dynamics during air exposure in total mixed ration (TMR) silage containing sweet potato residue. TMR were ensiled in laboratory silos (1 kg) with or without two lactic acid bacteria strains, Lactobacillus plantarum (LP), and Lactobacillus amylovorus (LA). Fermentation characteristics were measured and yeast population was investigated by ITS1 region gene sequencing using Illumina MiSeq platform. All treatments were well ensiled, and L. amylovorus improved aerobic stability. During aerobic exposure, Pichia kudriavzevii was detected with increased relative abundance in all treatments and more relative abundant in LP. Pichia fermentans was more relative abundant in control. Higher relative abundance of Pichia anomala was detected in deteriorating LP. The relative abundance of Pichia ohmeri increased during later aerobic exposure in the control and LA, with a significant increase in the count of yeast population. Despite Cryptococcus was detected more relative abundant during early stage of aerobic exposure, the yeast population was below the detection limit. Aerobic deterioration was characterized by an increase in operational taxonomic units of Pichia. High relative abundance of P. anomala and P. kudriavzevii made aerobic deterioration easier. Inhibition of P. fermentans might be an effective strategy for improving the aerobic stability to some instance.
为了研究含有甘薯渣的全混合日粮（TMR）青贮在暴露于空气中时酵母种群的动态变化。将 TMR 在实验室青贮窖（1kg）中进行青贮，分别添加或不添加两种乳酸菌菌株，植物乳杆菌（LP）和淀粉乳杆菌（LA）。通过 Illumina MiSeq 平台使用 ITS1 区基因测序来测量发酵特性并研究酵母种群。所有处理均得到良好青贮，添加淀粉乳杆菌可提高有氧稳定性。在有氧暴露期间，所有处理中均检测到毕赤酵母属的相对丰度增加，且 LP 中相对丰度更高。在对照中发酵毕赤酵母属的相对丰度更高。在恶化的 LP 中，异常毕赤酵母属的相对丰度更高。在控制和 LA 中，在后期有氧暴露过程中，假丝酵母属的相对丰度增加，酵母种群的数量显著增加。尽管在有氧暴露的早期阶段，隐球菌属的相对丰度更高，但酵母种群的数量低于检测限。有氧变质的特征是毕赤酵母属的分类单元数量增加。异常毕赤酵母属和毕赤酵母属的高相对丰度使得有氧变质更容易。抑制发酵毕赤酵母属可能是提高某些情况下有氧稳定性的有效策略。
