Fisheries College, Jimei University, Xiamen, 361021, China; Inspection and Quarantine Technology Center of Cross-Strait Agricultural Products in Xiamen, Xiamen, 361021, China.
Inspection and Quarantine Technology Center of Cross-Strait Agricultural Products in Xiamen, Xiamen, 361021, China.
Mol Cell Probes. 2020 Oct;53:101609. doi: 10.1016/j.mcp.2020.101609. Epub 2020 May 30.
Increasingly globalized and complex food supply chains contribute to a growing problem of meat fraud. Meat adulteration with pork is especially exceptionable to the global population for health concern and religious faith reasons. To prevent unfair competition and protect consumer rights, an efficient and rapid assay to identify the species of meat products is crucial. In this study, a real-time loop-mediated isothermal amplification (real-time LAMP) assay was developed for the detection of a porcine gene in meat products. The designed primers were highly selective for the porcine gene. The amplification showed no cross-reactivity with 11 other meats. The established method required 20 min with an initial amplification curve of approximately 10 min and demonstrated a detection limit of 1.76 pg/μL porcine DNA, which is 1000 times more sensitive than PCR. This study is the first attempt at detecting porcine-derived ingredients using a real-time LAMP assay in commercial products. This method meets specificity, rapidness, robustness, and sensitivity criteria; its practical application will greatly aid in battling adulteration in the food industry.
日益全球化和复杂的食品供应链导致肉类掺假问题日益严重。由于健康问题和宗教信仰原因，肉类掺假猪肉在全球范围内尤其令人反感。为了防止不公平竞争和保护消费者权益，快速准确地识别肉类产品的物种至关重要。本研究开发了一种用于检测肉类产品中猪基因的实时环介导等温扩增（real-time LAMP）检测方法。设计的引物对猪基因具有高度选择性。扩增对其他 11 种肉类没有交叉反应。该方法的建立需要 20 分钟，初始扩增曲线约为 10 分钟，检测限为 1.76pg/μL 猪 DNA，比 PCR 灵敏 1000 倍。本研究首次尝试使用实时 LAMP 检测试剂盒检测商业产品中的猪源性成分。该方法符合特异性、快速性、稳健性和敏感性标准；其实际应用将极大地帮助打击食品工业中的掺假行为。
