Reactions of cisplatin and cis-[PtI(NH)] with molecular models of relevant protein sidechains: A comparative analysis.

Quite surprisingly, cisplatin and cis-[PtI(NH)] were found to manifest significant differences in their reactions with the model protein lysozyme. We decided to explore whether these differences recur when reacting these two Pt compounds with other proteins. Notably, ESI-MS measurements carried out on cytochrome c nicely confirmed the reaction pattern observed for lysozyme. This prompted us to exploit a computational DFT approach to disclose the molecular basis of such behavior. We analyzed comparatively the reactions of cis-[PtCl(NH)] and cis-[PtI(NH)] with appropriate molecular models (Ls) of the sidechains of relevant aminoacids. We found that when Pt(II) complexes are reacted with sulfur ligands both quickly lose their halide ligands and then the resulting cis-[Pt(L)(NH)] species loses ammonia upon reaction with a ligand excess. In the case of imidazole, again cis-[PtCl(NH)] and cis-[PtI(NH)] quickly lose their halide ligands but the resulting cis-[Pt(L)(NH)] species does not lose ammonia by reaction with excess imidazole. These results imply that the two platinum complexes manifest a significantly different behavior in their reaction with representative small molecules in agreement with what observed in the case of model proteins. It follows that the protein itself must play a crucial role in triggering the peculiar reactivity of cis-[PtI(NH)] and in governing the nature of the formed protein adducts. The probable reasons for the observed behavior are critically commented and discussed.