Department of Pediatric pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands.
Department of Pediatric pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands.
J Cyst Fibros. 2020 Jul;19(4):553-561. doi: 10.1016/j.jcf.2020.04.007. Epub 2020 May 30.
Patients with Cystic Fibrosis (CF) suffer from pancreatic insufficiency, lipid malabsorption and gastrointestinal complaints, next to progressive pulmonary disease. Altered mucosal homoeostasis due to malfunctioning chloride channels results in an adapted microbial composition of the gastrointestinal and the respiratory tract. Additionally, antibiotic treatment has the potential to distort resident microbial communities dramatically. This study aims to investigate early life development of the gut microbial community composition of children with CF compared to healthy infants and to study the independent effects of antibiotics taking into account other clinical and lifestyle factors.
Faecal samples from 20 infants with CF and 45 healthy infants were collected regularly during the first 18 months of life and microbial composition was determined using 16S rRNA based sequencing.
We observed significant differences in the overall microbiota composition between infants with CF and healthy infants (p<0.001). Akkermansia and Anaerostipes were significantly more abundant in control infants, whereas Streptococci and E. coli were significantly more abundant in infants with CF, also after correction for several clinical factors (p<0.05). Antibiotic use in infants with CF was associated with a lower alpha diversity, a reduced abundance of Bifidobacterium and Bacteroides, and a higher abundance of Enterococcus.
Microbial development of the gut is different in infants with CF compared to healthy infants from the first months of life on, and further deviates over time, in part as a result of antibiotic treatment. The resulting dysbiosis may have significant functional consequences for the microbial ecosystem in CF patients.
囊性纤维化（CF）患者除了进行性肺部疾病外，还患有胰腺功能不全、脂肪吸收不良和胃肠道问题。由于氯离子通道功能障碍导致的黏膜稳态改变，导致胃肠道和呼吸道的微生物组成发生适应性改变。此外，抗生素治疗有可能显著改变定植微生物群落。本研究旨在比较 CF 患儿与健康婴儿的早期肠道微生物群落组成，并研究抗生素的独立作用，同时考虑其他临床和生活方式因素。
收集 20 名 CF 患儿和 45 名健康婴儿在生命的前 18 个月内定期的粪便样本，并使用基于 16S rRNA 的测序技术来确定微生物组成。
我们观察到 CF 患儿和健康婴儿之间的总体微生物群落组成存在显著差异（p<0.001）。阿克曼氏菌和厌氧棒状菌在对照组婴儿中明显更丰富，而链球菌和大肠杆菌在 CF 患儿中明显更丰富，即使在纠正了几个临床因素后也是如此（p<0.05）。CF 患儿的抗生素使用与较低的 alpha 多样性、双歧杆菌和拟杆菌丰度降低以及肠球菌丰度增加有关。
从生命的第一个月开始，CF 患儿的肠道微生物发育与健康婴儿不同，并且随着时间的推移进一步偏离，部分原因是抗生素治疗。由此产生的生态失调可能对 CF 患者的微生物生态系统产生重大功能影响。
