转移性乳腺癌患者泪囊体中的致癌 miRNA 鉴定。

Oncogenic miRNAs Identified in Tear Exosomes From Metastatic Breast Cancer Patients.

机构信息

Division of Radiation Oncology, Kobe University Graduate School of Medicine, Hyogo, Japan.

AntiCancer Inc, San Diego, CA, U.S.A.

出版信息

Anticancer Res. 2020 Jun;40(6):3091-3096. doi: 10.21873/anticanres.14290.

DOI:10.21873/anticanres.14290

PMID:32487603

Abstract

BACKGROUND/AIM: Exosomes are produced by normal and cancer cells. Exosomes are found in the serum of cancer patients and have been used for diagnosis and prognosis. Recently tears from non-cancer patients have been found to contain exosomes. In the present report we describe tears from advanced breast-cancer patients.

MATERIALS AND METHODS

We found oncogenic miRNAs in the exosomes isolated from tear fluids obtained from five patients with metastatic breast cancer and compared them with tear exosomes form eight healthy volunteers.

RESULTS

Tear exosomes had a significantly higher quantity of exosome markers than serum exosomes (CD9, CD63). Tear exosomes were subjected to quantitative reverse-transcription polymerase reaction (qRT-PCR), and western blot analysis to elucidate the status of miRNAs, previously reported in serum from patients with metastatic breast cancer. qRT-PCR and western-blot analysis revealed that breast-cancer-specific miR-21 and miR-200c were highly expressed in tear exosomes from metastatic breast cancer patients in contrast to tear exosomes from healthy volunteers.

CONCLUSION

Tear exosomes can be a potential source of diagnostic and prognostic biomarkers for metastatic breast cancer, and possibly other cancers or diseases.

摘要

背景/目的：外泌体由正常细胞和癌细胞产生。外泌体存在于癌症患者的血清中，已被用于诊断和预后。最近，在非癌症患者的泪液中也发现了外泌体。在本报告中，我们描述了来自晚期乳腺癌患者的泪液中的外泌体。

材料和方法

我们从五名转移性乳腺癌患者的泪液中分离出外泌体，发现了致癌 miRNA，并将其与来自 8 名健康志愿者的泪液外泌体进行了比较。

结果

泪液外泌体的外泌体标志物（CD9、CD63）数量明显高于血清外泌体。对泪液外泌体进行定量逆转录聚合酶链反应（qRT-PCR）和 Western blot 分析，以阐明先前在转移性乳腺癌患者血清中报道的 miRNA 的状态。qRT-PCR 和 Western blot 分析显示，与健康志愿者的泪液外泌体相比，转移性乳腺癌患者的泪液外泌体中高度表达乳腺癌特异性的 miR-21 和 miR-200c。