Liu Pei-Wei, Lyu Fei-Fei, Zhang Yu-Xiu, Yang Yun, Gao Zhi-Hui, Liang Hui-Hui, Wei Jian-He
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Haikou 570311, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2020 Apr;45(7):1641-1647. doi: 10.19540/j.cnki.cjcmm.20200205.107.
Aquilaria sinensis is a typical inducible medicinal plant, that can produce agarwood only after it is wounded by external stimuli. Alternative oxidase(AOX) is one of the terminal oxidases of the plant mitochondrial electron transport, which plays an important role in plants' response to environmental stress. In order to reveal the physiological function of AOX gene in the process of agarwood formation from A.sinensis induced by wounding, AOX gene was cloned based on the transcriptome database and then identified by the bioinformatics analysis, and their expression pattern in different tissues and under wounding stress were detected by qRT-PCR. The results as follows. Three AOX genes were cloned from A.sinensis for the first time. They were named AsAOX1a, AsAOX1d and AsAOX2, respectively. The tissue expression shown that AsAOX1a is mainly expressed in the stem and the seed, and the AsAOX1d and AsAOX2 genes are mainly expressed in the pulp and the stem. AsAOX1a and AsAOX1d genes are highly responsive to wounding stress, and their response time was different. In addition, the expression of AsAOX1a and AsAOX2 induced by wounding are reduced by H_2O_2 treatment, but promoted by AsA treatment. The cloning, bioinformatics analysis and expression characteristics of AOX genes from A.sinensis provided basic information for further study the function of AOX genes in the development of A.sinensis, especially in the process of agarwood formation of A. sinensis induced by wounding.
白木香是一种典型的诱导型药用植物，只有在受到外界刺激受伤后才能产生沉香。交替氧化酶（AOX）是植物线粒体电子传递的末端氧化酶之一，在植物对环境胁迫的响应中起重要作用。为了揭示AOX基因在白木香受伤诱导形成沉香过程中的生理功能，基于转录组数据库克隆了AOX基因，然后通过生物信息学分析进行鉴定，并通过qRT-PCR检测其在不同组织和受伤胁迫下的表达模式。结果如下。首次从白木香中克隆到3个AOX基因，分别命名为AsAOX1a、AsAOX1d和AsAOX2。组织表达显示，AsAOX1a主要在茎和种子中表达，AsAOX1d和AsAOX2基因主要在果肉和茎中表达。AsAOX1a和AsAOX1d基因对受伤胁迫高度响应，但其响应时间不同。此外，受伤诱导的AsAOX1a和AsAOX2的表达在H_2O_2处理下降低，但在AsA处理下升高。白木香AOX基因的克隆、生物信息学分析及表达特性为进一步研究AOX基因在白木香发育，尤其是受伤诱导白木香形成沉香过程中的功能提供了基础信息。
