Li Xiang, Tian Dongping, Guo Yi, Qiu Shiyue, Xu Zexin, Deng Wen, Su Min
Institute of Clinical Pathology, Guangodng Provincial Key Laboratory of Infectious Disease and Molecular Immunopathology, Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong Province 515041 People's Republic of China.
The Judicial Critical Center, Shantou University Medical College, Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong Province 515041 People's Republic of China.
Cancer Cell Int. 2020 May 24;20:184. doi: 10.1186/s12935-020-01268-x. eCollection 2020.
Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. Misidentifying and cross-contaminations of cell lines also hamper the way of producing solid and reproductive data.
CSEC216 was originated from a 45-year-old male ESCC patient from Chaoshan littoral, China. Specimens were minced into fragments and seeded in T-25 flask for primary culture. Immunoflourescence staining was performed for identifying the origination and proliferation activity. In vitro migration and invasion abilities was tested by transwell assay. DNA Short Tandem Repeats profiling was implemented for cell authorization. Karyotype was investigated by spectrum karyotyping. Whole genome sequencing was utilized to investigate genomic alterations. Background information and genomic mutation data of published ESCC cell lines were obtained from online databases.
CSEC216 was an uncontaminated cell line, exhibited epithelial cell features with polygonal morphology and adherent growth as monolayer. Immuno staining demonstrated its epithelial origination and high proliferation rate. The Population Doubling time was 29.7 h. The karyotype demonstrated tumor cell patterns with aneuploidy and complex chromosomal aberrations. Mutation signatures, genes with SNA or CNA of CSEC216 and published ESCC cell lines were similar with the mutation spectrum of original ESCC tumors.
ESCC cell line CSEC216 from high incidence region in China was established with no cross-contamination. Biological features were studied. Genomic mutation features of CSEC216 and 28 ESCC cell lines were characterized which provided thorough cytogenetic background that facilitated future usage.
食管鳞状细胞癌（ESCC）是全球最常见的恶性肿瘤之一，也是癌症相关死亡的主要原因，在中国尤为如此。细胞系是基础医学研究中广泛使用的疾病模型，然而，来自中国的特征明确的ESCC细胞模型鲜有报道。细胞系的错误鉴定和交叉污染也阻碍了可靠和可重复数据的产生。
CSEC216源自一名来自中国潮汕沿海地区的45岁男性ESCC患者。将标本切碎成小块，接种于T-25培养瓶中进行原代培养。进行免疫荧光染色以鉴定其来源和增殖活性。通过Transwell实验检测体外迁移和侵袭能力。采用DNA短串联重复序列分析进行细胞鉴定。通过光谱核型分析研究核型。利用全基因组测序研究基因组改变。已发表的ESCC细胞系的背景信息和基因组突变数据从在线数据库中获取。
CSEC216是一个未受污染的细胞系，呈现上皮细胞特征，形态为多边形，以单层贴壁生长。免疫染色显示其上皮来源和高增殖率。群体倍增时间为29.7小时。核型显示为肿瘤细胞模式，具有非整倍体和复杂的染色体畸变。CSEC216和已发表的ESCC细胞系的突变特征、具有SNA或CNA的基因与原始ESCC肿瘤的突变谱相似。
建立了来自中国高发地区的ESCC细胞系CSEC216，无交叉污染。研究了其生物学特性。对CSEC216和28个ESCC细胞系的基因组突变特征进行了表征，提供了全面的细胞遗传学背景，便于未来使用。
