白化兔玻璃体内注射含万古霉素纳米脂质体的制备与评价
Preparation and evaluation of nanoliposomes containing vancomycin after intravitreal injection in albino rabbits.
作者信息
Abrishami Majid, Motamed Shariati Mehrdad, Malaekeh-Nikouei Bizhan, Tajani Amineh Sadat, Mahmoudi Asma, Abrishami Mojtaba, Khameneh Bahman
机构信息
Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Retina Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Basic Med Sci. 2020 Apr;23(4):551-555. doi: 10.22038/ijbms.2020.43447.10205.
OBJECTIVES
The efficacy of nanoliposomal formulation of vancomycin against methicillin-resistant (MRSA) assessed.
MATERIALS AND METHODS
Nanoliposomal formulations were prepared and characterized. The study was carried out on rabbits which received liquid culture medium containing MRSA under anesthesia. After 48 hr, the eyes treated with the liposomal and free form of vancomycin. The rabbits were euthanized at predesignate intervals at 12, 24, 48, 96, 144 hr intervals injection. The antibacterial activity of different vancomycin formulations was assayed by the time killing method.
RESULTS
The zeta potential, mean sizes and encapsulation efficacy of liposomal vancomycin were 29.7 mV, 381.93±30.13 nm and 47%, respectively. The results of time-killing studies indicated that the liposomal formula was more effective than the free form of vancomycin.
CONCLUSION
The results of this study revealed that liposomal vancomycin formulation is a powerful nano-antibacterial agent to combat infectious endophthalmitis.
目的
评估万古霉素纳米脂质体制剂对耐甲氧西林金黄色葡萄球菌（MRSA）的疗效。
材料与方法
制备并表征纳米脂质体制剂。在麻醉状态下，对接受含MRSA液体培养基的家兔进行研究。48小时后，用脂质体形式和游离形式的万古霉素处理眼睛。在注射后的12、24、48、96、144小时等预先设定的时间间隔对家兔实施安乐死。采用时间杀菌法测定不同万古霉素制剂的抗菌活性。
结果
脂质体万古霉素的ζ电位、平均粒径和包封率分别为29.7 mV、381.93±30.13 nm和47%。时间杀菌研究结果表明，脂质体制剂比游离形式的万古霉素更有效。
结论
本研究结果表明，脂质体万古霉素制剂是一种对抗感染性眼内炎的强效纳米抗菌剂。
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