Portillo F, Pásaro R
Department of Animal Physiology, Faculty of Biology, University of Sevilla, Japan.
J Anat. 1988 Aug;159:11-8.
The location of bulbospinal neurons within the nucleus ambiguus with respect to laryngeal motoneurons has been studied by means of retrograde fluorescent neuronal markers (True Blue and Diamidino Yellow in rats, and Fast Blue and Diamidino Yellow in cats). One marker was injected into the cervical spinal cord, and the other into the intrinsic laryngeal muscles. Afterwards, the precise location of each neuronal pool was observed with the fluorescent microscope. The bulbospinal neurons in the rostral part of the nucleus were located ventrolaterally with respect to motoneurons, both in cats and rats; at more caudal levels of the nucleus the bulbospinal neurons were arranged ventromedially and ventrolaterally with respect to motoneurons in cats, and around the motoneurons in rats.
利用逆行荧光神经元标记物（大鼠用真蓝和双脒基黄，猫用快蓝和双脒基黄），研究了延髓疑核内球脊髓神经元相对于喉运动神经元的位置。一种标记物注入颈脊髓，另一种注入喉内肌。之后，用荧光显微镜观察每个神经元池的精确位置。在猫和大鼠中，疑核前部的球脊髓神经元相对于运动神经元位于腹外侧；在猫中，疑核更靠尾端的水平，球脊髓神经元相对于运动神经元排列在腹内侧和腹外侧，而在大鼠中则围绕运动神经元排列。
