El-Ghlban Samah, AbouElnour Elsayed Saber, El-Torgoman Abd El-Monem Abd El-Kader, Abu Elabas Saeed Mohamed Saeed
Biochemistry Division Department of Chemistry, Faculty of Science, El Menoufia University, Shebin El-Kom, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia University, Egypt.
Biochem Biophys Rep. 2019 Nov 26;22:100708. doi: 10.1016/j.bbrep.2019.100708. eCollection 2020 Jul.
Breast cancer is the most common invasive cancer and the leading cause of cancer death in women. The function of over a thousand genes is reported as affected by genetic modifications in breast cancer.
To study the gene expression of Epithelial Membrane 2 (EMP2) and β1-Integrin genes in patients with breast cancer.
This study was carried out by cooperation between the Biochemistry Division Department of Chemistry, Faculty of Science and Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University. This study included 120 subjects divided into 2 groups : Included 60 women with breast cancer undergoing modified radical mastectomy. Tissue specimens were taken from the cancerous breast tissue and from the marginal healthy breast tissues. : Included 60 age and sex-matched apparently healthy women served as a control group. All patients participants were subjected to full history taking, general clinical examination, abdominal ultrasound, CT-scan for abdomen, mammography, fine needle biopsy, histopathological examination, immunostaining of tissues, metastatic work up (chest x-ray and bone scan) and laboratory investigations including: Complete blood count (patients and controls), serum carbohydrate antigen 15-3 (patients and controls), detection of EMP2 and β1-Integrin genes expression in the tissue samples by formation of cDNA by reverse transcription PCR after RNA extraction and real-time PCR using SYBR Green technique.
Compared to healthy tissues, the breast cancer tissues had significant higher EMP2 and β1-Integringene expression levels. Also, there was a significant increase in CA15-3 in patients group as compared with the control group. It was found that EMP2 and β1-Integrin expression in malignant tissue samples correlates with advanced and metastatic disease.
The gene expression of EMP2 and β1-Integrin are important markers for the severity of breast cancer and they are good indicators of its prognosis.
乳腺癌是最常见的浸润性癌症，也是女性癌症死亡的主要原因。据报道，超过一千种基因的功能受乳腺癌基因修饰的影响。
研究上皮膜蛋白2（EMP2）和β1整合素基因在乳腺癌患者中的基因表达。
本研究由曼苏拉大学理学院化学系生物化学教研室与医学院医学生物化学与分子生物学系合作开展。本研究纳入120名受试者，分为2组：包括60例行改良根治性乳房切除术的乳腺癌女性。从癌性乳腺组织和边缘健康乳腺组织采集组织标本。包括60名年龄和性别匹配的明显健康女性作为对照组。所有参与患者均进行了详细病史采集、全身临床检查、腹部超声、腹部CT扫描、乳房X线摄影、细针活检、组织病理学检查、组织免疫染色、转移灶检查（胸部X线和骨扫描）以及实验室检查，包括：全血细胞计数（患者和对照组）、血清糖类抗原15-3（患者和对照组），在提取RNA后通过逆转录PCR形成cDNA并使用SYBR Green技术进行实时PCR，检测组织样本中EMP2和β1整合素基因的表达。
与健康组织相比，乳腺癌组织中EMP2和β1整合素基因表达水平显著更高。此外，患者组CA15-3水平较对照组显著升高。发现恶性组织样本中EMP2和β1整合素的表达与晚期和转移性疾病相关。
EMP2和β1整合素的基因表达是乳腺癌严重程度的重要标志物，也是其预后的良好指标。
