Cook Sophie R, Badell-Grau Rafael A, Kirkham Emily D, Jones Kimberley M, Kelly Brendan P, Winston Jincy, Waller-Evans Helen, Allen Nicholas D, Lloyd-Evans Emyr
School of Biosciences, Sir Martin Evans Building, Cardiff University, Cardiff, CF10 3AX, UK.
Medicines Discovery Institute, Main Building, Cardiff University, Cardiff, CF10 3AT, UK.
AMRC Open Res. 2020 May 18;2:21. doi: 10.12688/amrcopenres.12903.1.
Good's buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced pluripotent stem cell-derived neurons, this study explores the effect of zwitterionic buffers, specifically HEPES, on lysosomal volume and Ca levels. Certain zwitterionic buffers lead to lysosomal expansion and reduced lysosomal Ca. Care should be taken when selecting buffers for growth media to avoid detrimental impacts on lysosomal function.
Good缓冲液常用于细胞培养，尽管其设计初衷是使其对生物学的影响最小甚至没有影响，但它们会引起细胞过程的改变，如自噬和溶酶体酶活性。本研究使用中国仓鼠卵巢细胞和诱导多能干细胞衍生的神经元，探讨两性离子缓冲液，特别是HEPES，对溶酶体体积和钙水平的影响。某些两性离子缓冲液会导致溶酶体扩张并降低溶酶体钙含量。在为生长培养基选择缓冲液时应谨慎，以避免对溶酶体功能产生不利影响。
