Shokrzadeh Mohammad, Goleij Pouya, Behravan Elmira, Ghassemi-Barghi Nasrin, Salehabadi Yaser, Rezaei Abolhasan
Mazandaran University of Medical Sciences, Faculty of Pharmacy, Department of Toxicology, Sari, Iran.
Sana Institute of Higher Education, Faculty of Biology, Department of Genetics, Sari, Iran.
Arq Gastroenterol. 2020 Apr-Jun;57(2):137-143. doi: 10.1590/s0004-2803.202000000-25.
Intestinal cancer often occurs in type 2 diabetic patients. The concept of increasing insulin levels and insulin-like growth factor in the blood with type 2 diabetes are stimulated with the growth and depletion of cloned cell walls, and the continuation of this process leads to the cellular deformation. This is the evidence for intestinal cancer in type 2 diabetes in population.
In this study, we aimed to find out the relationship between diabetics and intestinal cancer based on CD38 gene mutation.
Samples were collected from 200 population including normal and case ones. PCR products related to rs 6449181 of CD38 gene was amplified with ARMS-PCR technique, and a 420-bp sharp banding was observed as well. According three ARMS-PCR techniques, three primers were designed by oligo7 software. Primers include F1, F2 and R (amplifying for normal, mutant and reverse primer respectively).
This band was observed using a primer F1 that carries the wild type nucleotide using a primer, and when it is used with the F2 primer, it brings the mutant primer to populations of patients with diabetes and diabetes-cancer. In addition, the clinical results including body mass index, blood glucose and insulin level were analyzed. The means ±SD and Tuckey's post hoc test were significant between the clinical characterization parameters between cases and healthy populations. The allelic gene frequencies and Hardy-Weinberg equilibrium between nucleotides were evaluated, and the significant level between the alleles and gene frequencies was observed.
In general, the current study found that there is a relationship between diabetes and intestinal cancer among the studied populations.
肠道癌常发生于2型糖尿病患者中。2型糖尿病患者血液中胰岛素水平和胰岛素样生长因子的增加概念，会随着克隆细胞壁的生长和消耗而受到刺激，这一过程的持续会导致细胞变形。这是人群中2型糖尿病发生肠道癌的证据。
在本研究中，我们旨在基于CD38基因突变找出糖尿病与肠道癌之间的关系。
从200名人群中采集样本，包括正常人和病例组。采用扩增阻滞突变系统聚合酶链反应（ARMS-PCR）技术扩增与CD38基因rs 6449181相关的PCR产物，同时观察到一条420bp的清晰条带。根据三种ARMS-PCR技术，使用oligo7软件设计了三条引物。引物包括F1、F2和R（分别用于扩增野生型、突变型和反向引物）。
使用携带野生型核苷酸的引物F1观察到该条带，当它与F2引物一起使用时，在糖尿病患者和糖尿病合并癌症患者群体中出现突变引物。此外，分析了包括体重指数、血糖和胰岛素水平在内的临床结果。病例组和健康人群之间临床特征参数的均值±标准差及Turkey事后检验具有显著性。评估了核苷酸之间的等位基因频率和哈迪-温伯格平衡，并观察到等位基因和基因频率之间的显著水平。
总体而言,本研究发现在所研究人群中糖尿病与肠道癌之间存在关联。
