Kretova O V, Fedoseeva D M, Slovohotov I Y, Klushevskaya E S, Kravatsky Y V, Tchurikov N A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2020 May-Jun;54(3):445-449. doi: 10.31857/S0026898420020081.
In experiments on mouse and human cells it was demonstrated that rDNA plays an important role in epigenetic regulation of many genes. To identify and study rDNA-contacting genes in Drosophila we used the 4С (circular chromosome conformation capture) approach. We detected very stable contacts of rDNA genes within a 5-kb region inside the Tlk gene residing in X chromosome. This 5-kb region corresponds to small RNAs. After heat shock treatment both the amount of contacts, and the expression level of the gene were increased. Tlk and Rala are genes that share the same short bidirectional promoter but exhibit different expression levels. Around the region of rDNA contacts inside the Tlk gene, looped domains were formed. We conclude that rDNA contact-dependent epigenetic regulation is guided by small RNAs and that the contacts are involved in rearrangements of the looped domains.
在对小鼠和人类细胞进行的实验中，已证明核糖体DNA（rDNA）在许多基因的表观遗传调控中发挥重要作用。为了鉴定和研究果蝇中与rDNA接触的基因，我们采用了4C（环状染色体构象捕获）方法。我们在位于X染色体上的Tlk基因内一个5kb区域内检测到rDNA基因的非常稳定的接触。这个5kb区域对应于小RNA。热休克处理后，接触量和基因表达水平均增加。Tlk和Rala是共享相同短双向启动子但表现出不同表达水平的基因。在Tlk基因内rDNA接触区域周围形成了环状结构域。我们得出结论，rDNA接触依赖性表观遗传调控由小RNA引导，并且这些接触参与环状结构域的重排。
