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通过蛋白质-绿原酸-葡聚糖共轭物作为乳化剂提高纳米乳液的稳定性和抗氧化性

The Improvement of Nanoemulsion Stability and Antioxidation via Protein-Chlorogenic Acid-Dextran Conjugates as Emulsifiers.

作者信息

Liu Chang, Jin Hua, Yu Yue, Sun Jingying, Zheng Huanyu, Zhang Yan, Xu Jing, Zhu Xiuqing

机构信息

College of Art and Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.

College of Food Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.

出版信息

Nanomaterials (Basel). 2020 Jun 1;10(6):1094. doi: 10.3390/nano10061094.

DOI:10.3390/nano10061094
PMID:32492859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7353371/
Abstract

In this experiment, the peanut protein isolate (PPI), soybean protein isolate (SPI), rice bran protein isolate (RBPI), and whey protein isolate (WPI) were modified by linking chlorogenic acid covalently and linking dextran by Maillard reaction to prepare protein-chlorogenic acid-dextran (PCD) conjugates. As for structures, conformational changes of conjugates were determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE), Fourier transform infrared (FT-IR), and fluorescence measurements. The molecular weights of PCD conjugates became larger, the structure became disorder, and the amino acid residues inside the protein were exposed to the polar environment when compared to protein-chlorogenic acid (PC) and native proteins (NPs). As for properties, the interfacial tension reduced and antioxidant activity of PCD conjugates enhanced in varying degrees. Based on this, PCD conjugates were used as emulsifiers in order to investigate the properties of nanoemulsions and compared with PC conjugates and NPs. The mean droplet diameters (MDD) results showed that the nanoemulsions that were stabilized by PCD conjugates had the smallest particle sizes and exhibited uniformly dispersed spherical shapes. The storage and oxidative stabilities of PCD conjugates were also significantly improved. In comparison, nanoemulsion that was stabilized by PPI-chlorogenic acid-dextran conjugate had the smallest particle size and optimal stability among four protein stabilized nanoemulsions.

摘要

在本实验中，通过将绿原酸共价连接并利用美拉德反应连接葡聚糖对花生分离蛋白（PPI）、大豆分离蛋白（SPI）、米糠分离蛋白（RBPI）和乳清分离蛋白（WPI）进行改性，以制备蛋白 - 绿原酸 - 葡聚糖（PCD）缀合物。至于结构方面，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）、傅里叶变换红外光谱（FT - IR）和荧光测量来确定缀合物的构象变化。与蛋白 - 绿原酸（PC）和天然蛋白（NPs）相比，PCD缀合物的分子量增大，结构变得无序，蛋白质内部的氨基酸残基暴露于极性环境中。至于性质方面，PCD缀合物的界面张力降低，抗氧化活性有不同程度的增强。基于此，将PCD缀合物用作乳化剂以研究纳米乳液的性质，并与PC缀合物和NPs进行比较。平均液滴直径（MDD）结果表明，由PCD缀合物稳定的纳米乳液具有最小的粒径，并呈现出均匀分散的球形。PCD缀合物的储存稳定性和氧化稳定性也显著提高。相比之下，在四种蛋白质稳定的纳米乳液中，由PPI - 绿原酸 - 葡聚糖缀合物稳定的纳米乳液具有最小的粒径和最佳的稳定性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/a31678fe85e4/nanomaterials-10-01094-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/f57b16e60e02/nanomaterials-10-01094-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/26db793960e5/nanomaterials-10-01094-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/6b51589bb117/nanomaterials-10-01094-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/9a13cfde20dd/nanomaterials-10-01094-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/58c69a7f8b3d/nanomaterials-10-01094-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/06115340ab74/nanomaterials-10-01094-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/694e243886a8/nanomaterials-10-01094-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/efb4907b3be2/nanomaterials-10-01094-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/a31678fe85e4/nanomaterials-10-01094-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/f57b16e60e02/nanomaterials-10-01094-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/26db793960e5/nanomaterials-10-01094-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/6b51589bb117/nanomaterials-10-01094-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/9a13cfde20dd/nanomaterials-10-01094-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/58c69a7f8b3d/nanomaterials-10-01094-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/06115340ab74/nanomaterials-10-01094-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/694e243886a8/nanomaterials-10-01094-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/efb4907b3be2/nanomaterials-10-01094-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b315/7353371/a31678fe85e4/nanomaterials-10-01094-g009.jpg

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