Zhang Yue, Deng Ai-Ping, Weng Qian-Qian, Lin Wang-Min, Zhan Zhi-Lai, Huang Lu-Qi
Henan University of Chinese Medicine Zhengzhou 450046, China National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(9):2130-2137. doi: 10.19540/j.cnki.cjcmm.20200219.202.
The purpose of this article is to study the degradation of chemical compositions after the silkworm excrement being expelled from the silkworm, and to determine its main metabolic compositions and their changing relationships. This research is based on UPLC-Q-TOF-MS technology. Based on the systematic analysis of the main chemical compositions contained in silkworm excrement, the principal compositions analysis(PCA) and partial least squares discriminant analysis(OPLS-DA) on commercial silkworm excrement and fresh silkworm excrement were analyzed for differences. The S-plot chart of OPLS-DA was used to select and identify the chemical compositions that contributed significantly to the difference. At the same time, the relative peak areas of the different compositions were extracted by Masslynx to obtain the relative content of different compositions in fresh silkworm excrement. The results showed that there was a significant difference in the chemical compositions between fresh silkworm excrement and commercial silkworm excrement. The difference compositions were mainly flavonoid glycosides and Diels-Alder type composition, and two types of compounds are degradated during the storage of silkworm sand. In this study, the chemical compositions of fresh silkworm excrement were systematically identified and analyzed for the first time by mass spectrometry, and it was found that some chemical compositions of silkworm excrement were degradated with time during storage.
本文旨在研究家蚕粪便排出后化学成分的降解情况，确定其主要代谢成分及其变化关系。本研究基于超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）技术。在对家蚕粪便中所含主要化学成分进行系统分析的基础上，对市售家蚕粪便和新鲜家蚕粪便进行主成分分析（PCA）和偏最小二乘判别分析（OPLS-DA）以分析差异。利用OPLS-DA的S-图来选择和鉴定对差异有显著贡献的化学成分。同时，通过Masslynx提取不同成分的相对峰面积，以获得新鲜家蚕粪便中不同成分的相对含量。结果表明，新鲜家蚕粪便和市售家蚕粪便的化学成分存在显著差异。差异成分主要为黄酮苷和双烯加成型成分，且这两类化合物在家蚕沙储存过程中会降解。在本研究中，首次通过质谱对新鲜家蚕粪便的化学成分进行了系统鉴定和分析，发现家蚕粪便的一些化学成分在储存过程中会随时间降解。
