Department of Orthopaedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xian, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(10):5691-5696. doi: 10.26355/eurrev_202005_21360.
To elucidate the role of Prunella vulgaris L (PVL) in protecting glucocorticoids (GC)-induced osteogenesis inhibition, thereafter, protecting the deterioration of osteoporosis (OP).
Cell Counting Kit-8 (CCK-8) assay was conducted to assess the influence of PVL treatment on MSCs viability. Osteogenesis in MSCs was induced by Dexamethasone (DEX) stimulation. Regulatory effects of PVL on osteogenesis-related gene expressions, ALP activity, and mineralization ability in DEX-induced MSCs were determined. At last, protein levels of p-Smad1/5/9 and total-Smad1/5/9 influenced by DEX and PVL were measured by Western blot.
PVL treatment did not pose a time- or dose-dependent influence on MSCs viability. DEX induction in MSCs downregulated ALP, RUNX2, Bglap, and Osterix. ALP activity and mineralization in DEX-induced MSCs were suppressed. Downregulated osteogenesis-related genes decreased ALP activity and mineralization in MSCs undergoing DEX stimulation were partially reversed by PVL treatment. Moreover, the downregulated p-Smad1/5/9 level in DEX-induced MSCs was elevated by PVL treatment, while total-Smad1/5/9 was not affected.
PVL alleviated GC-induced suppression in MSCs osteogenesis by activating the Smad pathway, thereafter, protecting the deterioration of OP.
阐明夏枯草（PVL）在保护糖皮质激素（GC）诱导的成骨抑制、进而保护骨质疏松（OP）恶化中的作用。
通过细胞计数试剂盒-8（CCK-8）测定法评估 PVL 处理对间充质干细胞（MSCs）活力的影响。用地塞米松（DEX）刺激诱导 MSCs 成骨。测定 PVL 对 DEX 诱导的 MSCs 中成骨相关基因表达、碱性磷酸酶（ALP）活性和矿化能力的调节作用。最后，通过 Western blot 测定 DEX 和 PVL 影响的 p-Smad1/5/9 和总-Smad1/5/9 蛋白水平。
PVL 处理对 MSCs 活力没有时间或剂量依赖性影响。DEX 诱导 MSCs 下调 ALP、RUNX2、Bglap 和 Osterix。DEX 诱导的 MSCs 中 ALP 活性和矿化受到抑制。下调的成骨相关基因使 DEX 刺激的 MSCs 中的 ALP 活性和矿化减少，PVL 处理部分逆转了这种情况。此外，DEX 诱导的 MSCs 中下调的 p-Smad1/5/9 水平被 PVL 处理上调，而总-Smad1/5/9 不受影响。
PVL 通过激活 Smad 通路缓解 GC 诱导的 MSCs 成骨抑制，进而保护 OP 的恶化。
