Shenzhen Hospital of Guangzhou University of Chinese Medicine (Futian), Shenzhen, China.
J Recept Signal Transduct Res. 2020 Dec;40(6):591-598. doi: 10.1080/10799893.2020.1772296. Epub 2020 Jun 4.
Tanshinone IIA (Tan IIA) is a member of the major lipophilic components extracted from the root of Bunge. Osteosarcomas are primary malignant tumors of bone. The aim of our study is to explore the role of Tan IIA in osteosarcomas survival, migration, and proliferation. MG63 osteosarcoma cell line was cultured and treated with different concentrations of Tan IIA. Then, ELISA, immunofluorescence, qPCR, western blots, and pathway analysis were conducted to verify whether Tan II modulated osteosarcoma survival, migration, and proliferation through the AMPK/Nrf2 signaling pathway. Our results indicated that Tan IIA dose-dependently inhibited MG63 osteosarcoma cell survival, migration, and proliferation. Mechanistically, Tan IIA reduced cell viability and inhibited the transcriptions of migratory factors. In addition, the number of proliferative MG63 osteosarcoma cell was also reduced by Tan IIA. Molecular investigations demonstrated that Tan IIA treatment caused a drop in the transcriptions and activities of AMPK and Nrf2. Interestingly, knockdown of AMPK and Nrf2 markedly attenuated MG63 osteosarcoma cell survival, migration, and proliferation. Altogether, our results indicate that Tan IIA could be used as an effective anticancer drug to control osteosarcoma proliferation through affecting its survival, migration, and proliferation.
丹参酮 IIA（Tan IIA）是从丹参根中提取的主要亲脂性成分之一。骨肉瘤是骨的原发性恶性肿瘤。我们的研究旨在探讨 Tan IIA 在骨肉瘤存活、迁移和增殖中的作用。培养 MG63 骨肉瘤细胞系并使用不同浓度的 Tan IIA 处理。然后，通过 ELISA、免疫荧光、qPCR、western blot 和通路分析来验证 Tan II 是否通过 AMPK/Nrf2 信号通路调节骨肉瘤的存活、迁移和增殖。我们的结果表明，Tan IIA 呈剂量依赖性抑制 MG63 骨肉瘤细胞的存活、迁移和增殖。在机制上，Tan IIA 降低了细胞活力并抑制了迁移因子的转录。此外，Tan IIA 还减少了增殖性 MG63 骨肉瘤细胞的数量。分子研究表明，Tan IIA 处理导致 AMPK 和 Nrf2 的转录和活性下降。有趣的是，AMPK 和 Nrf2 的敲低显著减弱了 MG63 骨肉瘤细胞的存活、迁移和增殖。总之，我们的结果表明，Tan IIA 可作为一种有效的抗癌药物，通过影响其存活、迁移和增殖来控制骨肉瘤的增殖。
