向去神经化的骨骼肌中注射中和脑源性神经营养因子（BDNF）和碱性成纤维细胞生长因子 2（FGF2）可改善神经修复后的恢复情况。
Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair.
机构信息
Department of Prosthetic Dentistry, School of Dental and Oral Medicine, University of Cologne, Germany.
Department of Anatomy I, University of Cologne, Germany.
出版信息
Muscle Nerve. 2020 Sep;62(3):404-412. doi: 10.1002/mus.26991. Epub 2020 Jun 18.
BACKGROUND
After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles.
METHODS
We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury.
RESULTS
Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude.
CONCLUSIONS
Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.
背景
在大鼠面神经损伤和修复后，触须的摆动恢复与高比例的单神经支配神经肌肉接头（NMJ）有关。我们之前对盲鼠（Sprague Dawley/皇家外科学院[RCS]）的研究表明，NMJ 单神经支配和触须摆动的自发性恢复与去神经化的触须肌肉中纤维母细胞生长因子-2（FGF2）和脑源性神经营养因子（BDNF）的增加有关。
方法
我们使用正常视力的大鼠（Wistar），其中 NMJ 多神经支配与触须摆动恢复不良高度相关，并在面神经损伤后的不同术后时期，向提上唇鼻翼肌（LLS）注射 BDNF、抗 BDNF 和 FGF2 的组合。
结果
接受抗 BDNF+FGF2 治疗的大鼠 NMJ 多神经支配较低，触须摆动幅度恢复最好。
结论
外周神经损伤后靶神经再支配的恢复需要一种复杂的、具有特定时间过程的营养因子混合物，每种因子都有其特定的可用性。
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