Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, People's Republic of China.
Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, People's Republic of China.
Int J Biol Macromol. 2020 Oct 1;160:795-805. doi: 10.1016/j.ijbiomac.2020.05.258. Epub 2020 Jun 1.
Silk fibroin (SF) is a promising biomaterial with attractive properties, however, the practical applications of pure regenerated SF materials were severely restricted by their poor water retention and low elasticity. In this study, a tyramine-modified hyaluronic acid (mHA) was introduced to SF chains, followed by constructing double-network hydrogels via laccase-mediated crosslinking of SF and mHA. Mechanism of the enzymatic coupling reaction between SF and mHA was investigated using the model compounds. The morphology, mechanical property, and swelling behavior of SF-g-mHA hydrogel were evaluated. The results reveal that combination use of SF and mHA endowed the obtained hydrogels with high structural stability, improved mechanical properties and drug release behavior, which overcame the intrinsically poor formability of the pure fibroin hydrogels. This work provides a novel method to construct a double-network hydrogel with high flexibility and toughness, and expands the application range of the SF-based materials in biomedical fields.
丝素蛋白（SF）是一种很有前途的生物材料，具有吸引人的特性，然而，由于其保水能力差和弹性低，纯再生 SF 材料的实际应用受到严重限制。在本研究中，将酪胺改性透明质酸（mHA）引入 SF 链中，然后通过漆酶介导的 SF 和 mHA 的交联构建双网络水凝胶。使用模型化合物研究了 SF 和 mHA 之间酶偶联反应的机制。评估了 SF-g-mHA 水凝胶的形态、力学性能和溶胀行为。结果表明，SF 和 mHA 的组合使用赋予了所得水凝胶高结构稳定性、改善的机械性能和药物释放行为，克服了纯丝素水凝胶固有的可成形性差的问题。这项工作提供了一种构建具有高柔韧性和韧性的双网络水凝胶的新方法，并扩展了基于 SF 的材料在生物医学领域的应用范围。
