Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
Talanta. 2020 Sep 1;217:121098. doi: 10.1016/j.talanta.2020.121098. Epub 2020 May 1.
β-Galactosidase (β-gal) is an important biomarker for primary ovarian cancers. Developing noninvasive bioimaging probes for studying the activity of β-gal is highly desirable for cancer diagnosis. Herein, a turn-on near-infrared (NIR) fluorescent probe， 2-((6-(((2S, 3R, 4S, 5R, 6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran -2-yl)oxy)-2,3-dihydro-1H-xanthen-4-yl)methylene)malononitrile named DXM-βgal, was rationally designed based on enzymatic reaction for the detection of β-gal activity both in vitro and in vivo. Upon incubating with β-gal, DXM-βgal displayed a significant fluorescence enhancement at 640 nm, accompanying by a color change of solution color from red to purple. DXM-βgal exhibited high selectivity and sensitively to β-gal with low limit of detection (2.92 × 10 U mL). Besides, based on its advantages of long-wavelength emission and excellent biocompatibility, DXM-βgal was successfully applied to imaging β-gal in living cells and zebrafish. Given these prominent properties, we believe that DXM-βgal will be a potential tool for investigating β-gal activity in biomedical research.
β-半乳糖苷酶（β-gal）是原发性卵巢癌的重要生物标志物。开发用于研究β-gal 活性的非侵入性生物成像探针对于癌症诊断非常有意义。在此，我们基于酶反应，设计了一种近红外（NIR）荧光探针 2-((6-(((2S, 3R, 4S, 5R, 6R)-3,4,5-三羟基-6-(羟甲基)四氢-2H-吡喃-2-基)氧基)-2,3-二氢-1H-呫吨-4-基)亚甲基)丙二腈，命名为 DXM-βgal，用于体外和体内检测β-gal 活性。与β-gal 孵育后，DXM-βgal 在 640nm 处显示出显著的荧光增强，同时溶液颜色从红色变为紫色。DXM-βgal 对β-gal 具有高选择性和敏感性，检测限低（2.92×10 U mL）。此外，基于其长波长发射和优异的生物相容性的优点，DXM-βgal 成功应用于活细胞和斑马鱼中β-gal 的成像。鉴于这些突出的特性，我们相信 DXM-βgal 将成为生物医学研究中研究β-gal 活性的潜在工具。
