School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 264209, China.
School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 264209, China; Weihai Chuanghui Environmental Protection Technology Co., Ltd, Weihai, 264200, China.
Talanta. 2020 Sep 1;217:121086. doi: 10.1016/j.talanta.2020.121086. Epub 2020 Apr 30.
SO derivatives maintain a certain balance and play an important role in many metabolic processes. However, excessive ingestion of them can lead to serious complications of various diseases. Therefore, a rapid and precision detection of SO derivatives with high selectivity and sensitivity would be an advance for their bio-analytic studies. Accordingly, a novel deep red and two-photon fluorescent SO derivatives probe (DRQ) was developed here for the first time. The probe showed fast ratio response rate (within 5 s), excellent sensitivity (the detection limit is 103 nM for red channel and 17 nM for green channel), and outstanding selectivity toward SO derivatives in 10 mM PBS buffer. Moreover, the ratiometric probe DRQ displays a 99 nm blue-shift in emission upon addition of SO derivatives. Intriguingly, the probe can be made into test papers for real-time monitoring of SO derivatives. Moreover, it can be also applied for visual ratio imaging of SO derivatives in cellular mitochondria and zebrafish under two-photon absorption (green channel) and one-photon absorption (red channel).
SO 衍生物在许多代谢过程中保持着某种平衡，发挥着重要作用。然而，过量摄入它们会导致各种疾病的严重并发症。因此，快速、精确地检测 SO 衍生物具有高选择性和灵敏度将是生物分析研究的一个进步。因此，我们首次开发了一种新型的深红光和双光子荧光 SO 衍生物探针 (DRQ)。该探针具有快速的比率响应速度（在 5s 内）、优异的灵敏度（红色通道的检测限为 103nM，绿色通道的检测限为 17nM），以及对 10mM PBS 缓冲液中的 SO 衍生物的出色选择性。此外，比率探针 DRQ 在加入 SO 衍生物后，发射出 99nm 的蓝移。有趣的是，该探针可以制成试纸，用于实时监测 SO 衍生物。此外，它还可以应用于细胞线粒体和斑马鱼中 SO 衍生物的双光子吸收（绿色通道）和单光子吸收（红色通道）的可视化比率成像。
