Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage (HUST), Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China.
Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage (HUST), Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China.
Talanta. 2020 Sep 1;217:121103. doi: 10.1016/j.talanta.2020.121103. Epub 2020 May 4.
A quick, highly selective and sensitive method for the determination of DNA was constructed based on the recovery of red fluorescence of carbon dots quenched by a ruthenium complex (Ru (bpy) (dppz), bpy = 2,2'-bipyridine; dppz = dipyrido [3.2-a:2',3'-c]phenazine). The carbon dots showed two emission peaks at 450 and 683 nm under excitation wavelength of 420 nm. After addition of Ru (bpy) (dppz), the fluorescence of carbon dots at 683 nm was markedly quenched. Due to the stronger interaction between DNA and Ru (bpy) (dppz), the quenched fluorescence of carbon dots was recovered with DNA added. Under the optimum conditions, there was a good linear relationship between the degree of fluorescence recovery of carbon dots and DNA concentration in range of 0.0150-9.60 μg mL. The detection limit was 0.00536 μg mL. The carbon dots were successfully applied to detect DNA in the simulated sample and the spiked recoveries were between 97.4% and 106%.
构建了一种基于红色荧光碳点（Ru（bpy）（dppz），bpy=2,2'-联吡啶；dppz=二吡啶并[3.2-a：2',3'-c]吩嗪）被钌配合物猝灭后恢复的快速、高选择性和高灵敏度测定 DNA 的方法。在 420nm 激发波长下，碳点在 450nm 和 683nm 处显示两个发射峰。加入 Ru（bpy）（dppz）后，碳点在 683nm 处的荧光明显猝灭。由于 DNA 与 Ru（bpy）（dppz）之间的相互作用更强，加入 DNA 后，猝灭的碳点荧光得以恢复。在最佳条件下，碳点荧光恢复程度与 DNA 浓度在 0.0150-9.60μg mL 范围内呈良好线性关系。检测限为 0.00536μg mL。碳点成功应用于模拟样品中 DNA 的检测，加标回收率在 97.4%至 106%之间。
