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冻存人卵母细胞和脐血细胞可产生具有纯合 HLA 类型的体细胞核移植衍生多能干细胞。

Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type.

机构信息

CHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Korea.

Graduated School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.

出版信息

Stem Cell Reports. 2020 Jul 14;15(1):171-184. doi: 10.1016/j.stemcr.2020.05.005. Epub 2020 Jun 4.

DOI:10.1016/j.stemcr.2020.05.005
PMID:32502464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7363744/
Abstract

Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.

摘要

人多能干细胞（PSCs）通过体细胞核移植（SCNT）可能成为再生医学的重要来源。干细胞的低衍生效率和人卵母细胞的可及性是其应用的主要障碍。我们之前报道过，通过过表达 H3K9me3 去甲基化酶可以提高 SCNT 的效率。在这里，我们将改良的衍生方法应用于 PSC 系，并首次从捐赠的冷冻保存卵母细胞和脐血细胞中获得了具有纯合人白细胞抗原（HLA）类型的人 SCNT-PSC 系。SCNT-PSC 与胚胎干细胞（ESCs）具有非常相似的特征，并且在具有匹配 HLA 类型的体外和体内人源化小鼠中表现出免疫相容性。我们的研究表明，使用具有已知 HLA 类型的捐赠冷冻保存卵母细胞和脐血细胞的 SCNT 技术为建立用于再生医学的人类 HLA 匹配 SCNT-PSC 库提供了一种有前途的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/76884c119225/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/eb17fcc82aea/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/2750fb49d19f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/2d8a4624d757/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/c48f631dd969/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/5d1442a98e6b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/b5e513894ec8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/76884c119225/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/eb17fcc82aea/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/2750fb49d19f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/2d8a4624d757/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/c48f631dd969/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/5d1442a98e6b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/b5e513894ec8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/118a/7363744/76884c119225/gr7.jpg

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