Centre for Technology in Water and Wastewater, University of Technology Sydney, Ultimo, NSW 2007, Australia.
School of Life and Environmental Sciences, University of Sydney, Camperdown, NSW 2006, Australia.
Bioresour Technol. 2020 Sep;312:123571. doi: 10.1016/j.biortech.2020.123571. Epub 2020 May 23.
This study evaluates the feasibility of a novel rumen membrane bioreactor (rumen MBR) to produce volatile fatty acids (VFA) from crop residues (i.e. lignocellulosic biomass). Rumen MBR can provide a sustainable route for VFA production by mimicking the digestive system of ruminant animals. Rumen fluid was inoculated in a reactor coupled with ultrafiltration (UF) membrane and fed with maize silage and concentrate feed at 60:40% (w/w). Continuous VFA production was achieved at an average daily yield of 438 mg VFA/g substrate. The most abundant VFA were acetic (40-80%) and propionic (10-40%) acids. The majority (73 ± 15%) of produced VFA was transferred through the UF membrane. Shifts in dominant rumen microbes were observed upon the transition from in vivo to in vitro environment and during reactor operation, however, stable VFA yield was maintained for 35 days, providing the first proof-of-concept of a viable rumen MBR.
本研究评估了新型瘤胃膜生物反应器（rumen MBR）从农作物残余物（即木质纤维素生物质）生产挥发性脂肪酸（VFA）的可行性。瘤胃 MBR 通过模拟反刍动物的消化系统，为 VFA 生产提供了一条可持续的途径。瘤胃液接种于与超滤（UF）膜耦合的反应器中，并以 60:40%（w/w）的比例进料玉米青贮和浓缩饲料。以 438 mg VFA/g 底物的平均日产量实现了连续 VFA 生产。最丰富的 VFA 是乙酸（40-80%）和丙酸（10-40%）。通过 UF 膜转移了大部分（73±15%）产生的 VFA。从体内到体外环境的转变以及在反应器运行期间观察到优势瘤胃微生物的变化，但 35 天内保持了稳定的 VFA 产量，为可行的瘤胃 MBR 提供了第一个概念验证。
