Department of Pharmacology, Rajarambapu College of Pharmacy, Kasegaon, 415404, India.
Department of Pharmacology, Appasaheb Birnale College of Pharmacy, Sangli -416416, India.
Comb Chem High Throughput Screen. 2021;24(3):415-422. doi: 10.2174/1386207323666200605150915.
The extracts and the compounds isolated from Phyllanthus amarus Schumm and Thonn (Family: Euphorbiaceae) have shown a wide spectrum of pharmacological activities including antiviral, antibacterial, antiplasmodial, antimalarial, antimicrobial, anticancer, antidiabetic, hypolipidemic, antioxidant, hepatoprotective, nephroprotective and diurectic properties.
This investigation was aimed at exploring the anxiolytic potential of Phyllanthus amarus standardized extracts and predict probable role of marker phyto constitutents.
Three standardized extracts of Phyllanthus amarus plant viz. standardized aqueous extract of Phyllanthus amarus whole plant (PAAE), standardized methanolic extract of P. amarus leaf (PAME) and the standardized hydro-methanolic extract of P. amarus leaf (PAHME) were tested in the classical animal models of anxiety: Elevated plus-maze model and Light & Dark Exploration test.
The lower doses of the tannin rich extract (PAHME) of the P. amarus possess significant anxiolytic activity compared to lignin rich (PAME) and aqueous extracts (PAAE), while at a higher dose (400mg/kg) the results of all three extracts appears to be potentially sedative. While the molecular docking studies support these probable anxiolytic, the sedative effects of the Phyllanthus amarus extracts could be due to the interaction of tannins and lignans with the GABAbenzodiazepine receptor complex.
The results of the present study indicate that the tannin-rich extract of the P. amarus may have potential clinical applications in the management of anxiety. It can be further studied for optimum dosage to be used as a future of anti-anxiety drug development or as a standardized Phytomedicine.
从叶下珠属植物（大戟科）中提取的提取物和分离得到的化合物具有广泛的药理活性，包括抗病毒、抗菌、抗疟原虫、抗疟疾、抗菌、抗癌、抗糖尿病、降血脂、抗氧化、保肝、保肾和利尿作用。
本研究旨在探索叶下珠标准化提取物的抗焦虑潜力，并预测可能的标记植物成分的作用。
对叶下珠植物的三种标准化提取物进行了测试，即叶下珠全植物标准化水提取物（PAAE）、叶下珠标准化甲醇提取物（PAME）和叶下珠叶标准化水-甲醇提取物（PAHME），采用经典的焦虑动物模型：高架十字迷宫模型和明暗探索试验。
富含单宁的叶下珠提取物（PAHME）的低剂量与富含木质素的提取物（PAME）和水提取物（PAAE）相比具有显著的抗焦虑活性，而在较高剂量（400mg/kg）时，三种提取物的结果似乎都具有潜在的镇静作用。虽然分子对接研究支持这些可能的抗焦虑作用，但叶下珠提取物的镇静作用可能是由于单宁和木脂素与 GABA-苯二氮䓬受体复合物的相互作用。
本研究结果表明，富含单宁的叶下珠提取物可能具有治疗焦虑症的临床应用潜力。可以进一步研究其最佳剂量，作为未来抗焦虑药物开发或作为标准化植物药的用途。
