夫妇在接受体外受精过程中的正常受精率和血清 25-OHD 水平：一项前瞻性队列研究。
Normal fertilisation rates and serum 25-OHD levels among couples undergoing in-vitro fertilisation: a prospective cohort study.
机构信息
Reproductive Medicine Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Reproductive Medicine Centre, First Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
BMC Pregnancy Childbirth. 2020 Jun 5;20(1):346. doi: 10.1186/s12884-020-02959-z.
BACKGROUND
The aim of this study was to explore the correlation between serum vitamin D levels in couples undergoing in-vitro fertilisation (IVF) and normal fertilisation process.
METHODS
Between March 2016 and March 2017, we performed a prospective cohort study at an academic reproductive medicine centre to measure serum 25-hydroxyvitaminD (25-OHD) levels of 1232 couples before controlled ovarian stimulation. Generalized linear regression and binary multivariate logistic regression were employed to assess whether 25-OHD levels in men and women correlated with normal fertilisation rates and low fertilisation rate (LFR).
RESULTS
Serum 25-OHD levels in women were classified into three groups: Group A, less than 10%; Group B, between 10 and 90%; and Group C, greater than 90%. Using generalized linear regression, we observed that female 25-OHD levels were related to normal fertilisation rates. Adjusted normal fertilisation rates from Group A to Group C in women were 59.50, 62.72, and 66.13%, respectively (P = 0.007). After binary logistic regression analysis, for women, compared with Group C, the ORs for LFR were 4.814 in Group A (95% CI, 1.266-18.309, P = 0.021) and were 3.204 in Group B (95% CI, 0.949-10.812, P = 0.061). Male 25-OHD levels were not related to the probability of low fertilisation rate (P > 0.05).
CONCLUSIONS
Circulating 25-OHD concentrations in women appear to be associated with normal fertilisation rates and low fertilisation rates in IVF cycles, but not in men. A further randomized controlled trial with vitamin D supplementation is needed to demonstrate whether female vitamin D levels exert an effect on the normal fertilisation process.
TRIAL REGISTRATION
https://clinicaltrials.gov/;NCT03305510; Registered 08 October 2017 - Retrospectively registered.
背景
本研究旨在探讨行体外受精（IVF）的夫妇血清维生素 D 水平与正常受精过程之间的相关性。
方法
2016 年 3 月至 2017 年 3 月，我们在一家学术生殖医学中心进行了一项前瞻性队列研究，以测量 1232 对夫妇在控制性卵巢刺激前的血清 25-羟维生素 D（25-OHD）水平。采用广义线性回归和二元多变量逻辑回归评估男性和女性的 25-OHD 水平是否与正常受精率和低受精率（LFR）相关。
结果
将女性的血清 25-OHD 水平分为三组：A 组，<10%；B 组，10%90%；C 组，>90%。通过广义线性回归，我们观察到女性 25-OHD 水平与正常受精率有关。女性 25-OHD 水平从 A 组到 C 组的调整后正常受精率分别为 59.50%、62.72%和 66.13%（P=0.007）。经过二元逻辑回归分析，与 C 组相比，A 组的 LFR 比值比（OR）为 4.814（95%可信区间[CI]，1.26618.309，P=0.021），B 组为 3.204（95%CI，0.949~10.812，P=0.061）。男性 25-OHD 水平与低受精率的概率无关（P>0.05）。
结论
女性循环 25-OHD 浓度似乎与 IVF 周期中的正常受精率和低受精率相关，但与男性无关。需要进一步的随机对照试验和维生素 D 补充来证明女性维生素 D 水平是否对正常受精过程有影响。
试验注册
https://clinicaltrials.gov/；NCT03305510；注册于 2017 年 10 月 8 日-回顾性注册。
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