Área de Oceanografía Química, Instituto Argentino de Oceanografía (IADO), CCT-CONICET, Camino La Carrindanga, km 7.5, Edificio E1, B8000FWB, Bahía Blanca, Buenos Aires, Argentina.
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), San Juan 670, B8000ICN, Bahía Blanca, Buenos Aires, Argentina.
Environ Sci Pollut Res Int. 2020 Sep;27(25):31905-31917. doi: 10.1007/s11356-020-09335-6. Epub 2020 Jun 6.
The detoxification process of trace metals in the estuarine burrowing crab Neohelice granulata, after previously being exposed to anthropogenic pressures in the field, is described for the first time. The objectives of this study were (a) to assess the metal content (Cd, Cu, Pb, Zn, Mn, Ni, Cr, Fe) in the sediments and the uptake of these elements in the hepatopancreas of N. granulata; (b) to quantify trace metal concentrations in the hepatopancreas before and after the detoxification experiment; and (c) to relate this information to metallothionein (MT) induction or reversibility. The detoxification assay was performed for 25 days with artificial seawater under controlled conditions in a culture chamber. The results showed higher uptake and bioaccumulation of Zn and Cu from the sediments, and the hepatopancreas exhibited increased levels of Zn and lower concentrations of the rest of the metals and MTs after the assay, mainly Fe and Mn that were significantly lower. We conclude that trace metals could be translocated to and accumulated in the hepatopancreas, the main metabolic organ, and then eliminated under controlled conditions with corresponding reversibility of MTs. Graphical abstract.
本研究首次描述了河口穴居蟹 Neohelice granulata 在经历野外人为压力后痕量金属的解毒过程。本研究的目的是：(a) 评估沉积物中的金属含量（Cd、Cu、Pb、Zn、Mn、Ni、Cr、Fe）和 Neohelice granulata 肝胰腺对这些元素的吸收；(b) 在解毒实验前后定量肝胰腺中的痕量金属浓度；以及 (c) 将这些信息与金属硫蛋白 (MT) 的诱导或可逆性联系起来。在培养室内的人工海水中进行了 25 天的解毒测定。结果表明，Neohelice granulata 从沉积物中吸收和生物积累了更多的 Zn 和 Cu，而肝胰腺在测定后显示 Zn 水平升高，其余金属和 MTs 的浓度降低，主要是 Fe 和 Mn 的浓度显著降低。我们得出结论，痕量金属可以被转运并积累在主要代谢器官肝胰腺中，然后在受控条件下被排出，相应的 MTs 具有可逆性。图表摘要。
