São Paulo State University (UNESP), Institute of Biosciences, Social Insect Study Center 24A Avenue 1515, Rio Claro, SP, Brazil.
São Paulo State University (UNESP), Institute of Biosciences, Social Insect Study Center 24A Avenue 1515, Rio Claro, SP, Brazil.
Environ Pollut. 2020 Oct;265(Pt B):114849. doi: 10.1016/j.envpol.2020.114849. Epub 2020 May 27.
Currently, Brazil has a full framework for pesticide risk assessment established for Apis mellifera, based on the North American approach. However, the use of Apis mellifera as model-organism as a surrogate for Brazilian native species of stingless bees has been questioned. Assessments on other stages of development than adult individual are essential. Our study aimed to standardize in vitro larval rearing method for the stingless bee species Scaptotrigona postica and Tetragonisca angustula, comparing the results to those obtained for M. scutellaris (previously described), for proposing the most suitable one for using in toxicological larval tests. We used the most efficient method for determining the toxicity of dimethoate on S. postica larvae. We presented the first comparative approach of responses to in vitro larval rearing methods among native bee species from Neotropical region, for use in risk assessment. Our results showed that S. postica was the most suitable native species to be proposed as model-organism. In addition, our results are also very useful for a ring test to validate the method, in accordance to OECD.
目前，巴西已经建立了一个完整的针对蜜蜂的农药风险评估框架，该框架基于北美方法。然而，将蜜蜂作为巴西本土无刺蜂的替代模式生物的使用一直受到质疑。对除成年个体以外的其他发育阶段进行评估是必不可少的。我们的研究旨在为无刺蜂物种 Scaptotrigona postica 和 Tetragonisca angustula 标准化体外幼虫饲养方法，并将结果与已描述的 M. scutellaris 进行比较，以提出最适合用于毒理学幼虫测试的方法。我们使用最有效的方法来确定乐果对 S. postica 幼虫的毒性。我们提出了在风险评估中，针对来自新热带地区的本地蜜蜂物种进行体外幼虫饲养方法的反应的首次比较方法。我们的结果表明，S. postica 是最适合作为模式生物的本地物种。此外，我们的结果对于根据经合组织进行的验证方法的环试验也非常有用。
