Esmaeilnejad Bijan, Tavassoli Mousa, Dalir-Naghadeh Bahram, Samiei Awat, Rajabi Sepideh, Mohammadi Vahid, Anassori Ehsan, Ehteshamfar Shahin
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Comp Immunol Microbiol Infect Dis. 2020 May 30;71:101503. doi: 10.1016/j.cimid.2020.101503.
Babesia bigemina infection (also known as Texas fever) is reported as the most prevalent and main causative agent of bovine babesiosis, worldwide. The current study was undertaken to assess indicators of oxidative stress including activities of antioxidant enzymes and total antioxidant capacity (TAC), oxidation status of biomolecules and serum levels of trace elements as well as indicators of inflammation including sialic acid (SA) contents and cholinesterase activity in cattle naturally infected with B. bigemina. An infected group comprised of 20 crossbred Holstein cattle (3-4 years old) were diagnosed to be positive by both microscopy and nested PCR assay. The infected animals were subdivided into two groups according to their parasitemia rates (<20 % and>20 %). Furthermore, 10 healthy cattle were included as the control. The infection caused severe anemia in a parasitemia-burden dependent fashion. The activities of catalase and glucose-6-phosphate dehydrogenase as well as the levels of TAC, zinc, selenium, copper and manganese were significantly decreased as the parasitemia increased, accordingly. However, the activity of superoxide dismutase as well as the levels of malondialdehyde, protein carbonylation, DNA damage and iron, were significantly elevated in a parasitemeia-burden dependent manner. Additionally, glutathione peroxidase activity was significantly elevated with the lower rate of parasitemia, but the higher rate had no significant effect as compared to control. Moreover, total, protein and lipid binding SA contents were significantly increased but the activities of acetylcholinesterase and butyrylcholinesterase were significantly reduced, parasitemia dependently. Conclusively, the infection was remarkably associated with the induction of anemia, oxidative stress and inflammation.
双芽巴贝斯虫感染（也称为得克萨斯热）据报道是全球范围内牛巴贝斯虫病最普遍且主要的病原体。本研究旨在评估自然感染双芽巴贝斯虫的牛的氧化应激指标，包括抗氧化酶活性和总抗氧化能力（TAC）、生物分子的氧化状态以及微量元素的血清水平，以及炎症指标，包括唾液酸（SA）含量和胆碱酯酶活性。一个感染组由20头杂交荷斯坦牛（3 - 4岁）组成，通过显微镜检查和巢式PCR检测均被诊断为阳性。感染动物根据其寄生虫血症发生率（<20%和>20%）分为两组。此外，纳入10头健康牛作为对照。感染以寄生虫血症负担依赖的方式导致严重贫血。随着寄生虫血症增加，过氧化氢酶和葡萄糖 - 6 - 磷酸脱氢酶的活性以及TAC、锌、硒、铜和锰的水平相应显著降低。然而，超氧化物歧化酶的活性以及丙二醛、蛋白质羰基化、DNA损伤和铁的水平以寄生虫血症负担依赖的方式显著升高。此外，谷胱甘肽过氧化物酶活性在较低寄生虫血症发生率时显著升高，但与对照相比，较高发生率时没有显著影响。而且，总、蛋白质和脂质结合的SA含量显著增加，但乙酰胆碱酯酶和丁酰胆碱酯酶的活性寄生虫血症依赖性地显著降低。总之，感染与贫血、氧化应激和炎症的诱导显著相关。
