电针对水回避应激小鼠核苷酸结合寡聚化结构域样受体家族含pyrin 结构域蛋白 6 表达的影响及其与肠易激综合征的关系。
Electroacupuncture relieves irritable bowel syndrome by increasing expression of nucleotide-binding oligomerization domain protein-like receptor family pyrin domain containing 6 in water-avoidance stress mice.
机构信息
Department of acupuncture, The Affiliated Hospital of Nanjing University of Chinese Meidicne, Nanjing 210029, China.
Department of the First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
出版信息
J Tradit Chin Med. 2020 Jun;40(3):407-413. doi: 10.19852/j.cnki.jtcm.2020.03.007.
OBJECTIVE
To investigate the effect of electroacupuncture (EA) on irritable bowel syndrome (IBS) in mice through regulating nucleotide-binding oligomerization domain protein-like receptor family pyrin domain containing 6 (NLRP6).
METHODS
Water-avoidance stress (WAS) mice model was used to investigate the effects and the mechanism of EA. Abdominal withdrawal reflex test, open field test, and intestinal motility test were used to evaluate visceral sensitivity, anxiety, and intestinal motility in mice. The expressions of NLRP6, Mucin-2 (MUC2) and E-cadherin were determined using immunofluorescence and Western blotting assays.
RESULTS
EA significantly upregulated the expression of NLRP6 in the intestine of mice. Moreover, EA increased the expressions of MUC2 and E-cadherin in WAS mice.
CONCLUSION
Our study found that the relief of IBS symptoms by EA may involve the increase in the expression of NLRP6 in WAS mice.
目的
通过调节核苷酸结合寡聚化结构域样受体家族富含 pyrin 结构域蛋白 6（NLRP6）研究电针对肠易激综合征（IBS）的影响。
方法
采用回避水应激（WAS）小鼠模型，观察电针对 WAS 模型的影响及其作用机制。通过腹壁退缩反射试验、旷场试验和肠运动试验评估小鼠内脏敏感性、焦虑和肠运动。采用免疫荧光和 Western blot 检测 NLRP6、黏蛋白-2（MUC2）和 E-钙黏蛋白的表达。
结果
电针显著上调了 WAS 小鼠肠组织中 NLRP6 的表达。此外，电针增加了 WAS 小鼠中 MUC2 和 E-钙黏蛋白的表达。
结论
本研究发现电针对 WAS 小鼠的缓解作用可能与 NLRP6 表达增加有关。
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