Li Jianbo, Hou Li, Sun Yi, Xing Jinyi, Jiang Yunliang, Kang Li
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
School of Life Science, Linyi University, Linyi 276000, China.
Anim Reprod Sci. 2020 Jul;218:106476. doi: 10.1016/j.anireprosci.2020.106476. Epub 2020 May 5.
The abundance of miR-1b-3p in the chicken ovary is greater after sexual maturation. In the present study there was assessment of whether a single nucleotine polymorphism (SNP) led to an alteration in expression of reproductive traits. The miR-1b-3p abundance was greatest in ovarian follicles The SNP site of rs737028527 (G > A), located in the 734 bp upstream region of pre-miR-1b-3p, was identified in three different chicken breeds. Results from an association analysis of chicken egg-laying traits indicated the SNP was associated with age at first egg production (AFE) and egg number at 32 and 48 weeks (E32, E48; P < 0.01). Hens with genotype AA had an earlier AFE and greater E32 and E48 than hens with other genotypes. The abundance of mature miR-1b-3p in the hens with the GG genotype was larger than those with the AA genotype (P < 0.01), and the luciferase activity of GG genotype promoter was also greater in birds with the AA than GG genotype (P < 0.05). There was inhibition of the production of the transcription factor bound by the specificity protein 1 (Sp1) as a result of the G-to-A mutation, and the luciferase activity of the GG, but not AA, genotype was markedly increased by Sp1. In conclusion, the SNP, rs737028527 (G> A), affected the abundance of mature miR-1b-3p by Sp1 and was associated with chicken egg-laying traits. Data from the present study allow for an increased understanding of the functions and regulation of miR-1b-3p in ovarian follicle development of hens.
性成熟后，鸡卵巢中miR-1b-3p的丰度更高。在本研究中，评估了单核苷酸多态性（SNP）是否导致生殖性状表达的改变。miR-1b-3p在卵巢卵泡中的丰度最高。在三个不同鸡品种中鉴定出位于pre-miR-1b-3p上游734 bp区域的rs737028527（G > A）SNP位点。鸡产蛋性状的关联分析结果表明，该SNP与初产蛋年龄（AFE）以及32周和48周的产蛋数（E32、E48；P < 0.01）相关。AA基因型的母鸡比其他基因型的母鸡AFE更早，E32和E48更高。GG基因型母鸡中成熟miR-1b-3p的丰度高于AA基因型母鸡（P < 0.01），并且AA基因型鸡中GG基因型启动子的荧光素酶活性也高于GG基因型（P < 0.05）。G到A的突变导致特异性蛋白1（Sp1）结合的转录因子产生受到抑制，并且Sp1可使GG基因型（而非AA基因型）的荧光素酶活性显著增加。总之，SNP rs737028527（G> A）通过Sp1影响成熟miR-1b-3p的丰度，并与鸡的产蛋性状相关。本研究的数据有助于加深对miR-1b-3p在母鸡卵巢卵泡发育中的功能和调控的理解。
