Long interval prostaglandin-based treatment regimens do not affect ovulatory or prolificacy rates of multiparous ewes after cervical fixed timed AI.

To evaluate effects of a longer, than conventional, interval between time of prostaglandin F (PG)-based administrations in a PG-based treatment regimen for fixed timed AI (FTAI) on ovulation rate (OR), non-estrous return rate on Day 21 subsequent to the time of AI (NRR21), as well as conception, prolificacy and fecundity rates, ewes were assigned to two groups. Ewes of treatment group (PG15) were estrous-synchronized using two PG doses 15 days apart and FTAI was conducted at 56 h after the second PG administration (Day 0). Ewes of the Control group (SE) had imposed a pre-estrous synchrony treatment regimen with two PG doses 7 days apart and AI was conducted after detection of spontaneous estrus from 17 to 19 days after the second PG administration (Day 0). Ovulation rate on Day 8, NRR21, conception, prolificacy and fecundity rates on Day 60 were evaluated. There were no differences (P > 0.05) between ewes of the PG15 and SE groups in OR (1.47 ± 0.50 and 1.54 ± 0.50, respectively) or prolificacy (1.42 ± 0.80 and 1.33 ± 0.62, respectively), however, there were lesser values (P< 0.05) in the PG15 than SE group for NRR21 (65.2% and 91.3%, respectively), conception (59.8% and 91.3%, respectively) and fecundity (84.8% and 120%, respectively). The longer interval with the PG-based treatment regimen does not affect OR and prolificacy, but there is a lesser NRR21, conception and fecundity rate in comparison to ewes of the Control group.