Posgrado en Producción Animal, Universidad Autónoma Chapingo.
Biocontrol Sci. 2020;25(2):107-112. doi: 10.4265/bio.25.107.
In this study, lactic acid bacteria (LAB) strains were isolated from ground beef, and it was analyzed if they have any effect on the growth of two reference bacteria (Salmonella sp. and Escherichia coli). It was found that five isolates showed an inhibitory effect in both reference bacteria by spot at the lawn assay. These bacteria were selected to perform growth kinetics in co-culture to determine if they modify the growth parameters of the reference bacteria. Subsequently, LAB cultures and three treatments (crude extract, thermally treated and thermally treated with neutral pH) of cells free supernatants (CFS) were screened by the agar well diffusion assay. In co-culture, selected LAB altered the growth rate and reduce the maximum population of both reference bacteria. While, LAB cultures and CFS also showed antimicrobial activity, and there was no significant difference among CFS treatments. LAB isolated from ground beef showed an antimicrobial effect against the reference bacteria that could be used for meat biopreservation purposes.
在本研究中，从碎牛肉中分离出乳酸菌（LAB）菌株，并分析它们对两种参考细菌（沙门氏菌和大肠杆菌）的生长是否有任何影响。发现有五个分离株在平板划线法中对两种参考细菌均具有抑制作用。选择这些细菌进行共培养的生长动力学研究，以确定它们是否改变参考细菌的生长参数。随后，通过琼脂孔扩散法筛选 LAB 培养物和细胞无细胞上清液（CFS）的三种处理（粗提取物、热处理和中性 pH 热处理）。在共培养中，选定的 LAB 改变了两种参考细菌的生长速率并降低了最大种群。而，LAB 培养物和 CFS 也显示出抗菌活性，并且 CFS 处理之间没有显著差异。从碎牛肉中分离出的 LAB 对参考细菌表现出抗菌作用，可用于肉类生物保鲜目的。
