Unit of Medical Pharmacy, Department of Pharmacy Practice, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, Nagasaki, 852-8588, Japan.
Anal Sci. 2020 Nov 10;36(11):1423-1426. doi: 10.2116/analsci.20P112. Epub 2020 Jun 5.
The identification of antigens incorporated into immune complexes (IC-antigens) is important for studying the pathophysiology of immunological diseases. Immune complexome analysis identifies IC-antigens by analyzing ICs collected from biological fluids by IC-capturing beads. In this study, we optimized the method to improve its comprehensiveness while maintaining selectivity for IC-antigens by comparing the number of identified peptides (model IC experiment) or proteins (human pooled serum) eluted from Protein G beads using different pH solutions (pH 2.0 - 11.0).
鉴定免疫复合物（IC）中包含的抗原对于研究免疫性疾病的病理生理学非常重要。免疫复合物组学分析通过分析用 IC 捕获珠从生物体液中收集的 IC 来鉴定 IC 抗原。在这项研究中，我们通过比较从 Protein G 珠洗脱的不同 pH 值溶液（pH 2.0-11.0）中鉴定出的肽（模型 IC 实验）或蛋白质（人混合血清）的数量，优化了该方法，在保持对 IC 抗原的选择性的同时提高了其全面性。
