Cheng Y D
Dalian Medical College.
Zhonghua Zhong Liu Za Zhi. 1988 Nov;10(6):402-5.
A cell line designated as Ca761-86 has been established from the solid mouse mammary cancer (Ca761) by suspension culture. It has been passaged for more than 200 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5'AMPase, ACP etc) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on Day 5 with mitotic index reaching 1.8% on Day 3. This cell line was a hyperdiploid with karyotype of 45 or 43, -2X, tri12, tri17, +M1-5. Cell agglutination was observed when treated with ConA (greater than or equal to 7 micrograms/ml). Spontaneous agglutination might also take place without adding any ConA. After 5 x 10(6) cells of Ca761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutaneous, intrafoot pad or intraperitoneal), the transplantability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca761-86 may be of value for practical purposes.
通过悬浮培养从实体小鼠乳腺癌（Ca761）建立了一种命名为Ca761 - 86的细胞系。它已传代200多代。以中等大小的圆形细胞为主，大多数为单核细胞。通过电子显微镜观察到了恶性细胞和A型病毒样颗粒的一些特征。细胞化学研究（DNA、RNA、SDH、5'AMP酶、ACP等）的结果与超微结构结果相当。它在第5天增殖约27.4倍，第3天有丝分裂指数达到1.8%。该细胞系为超二倍体，核型为45或43，-2X，tri12，tri17，+M1 - 5。用刀豆蛋白A（≥7微克/毫升）处理时观察到细胞凝集。不添加任何刀豆蛋白A时也可能发生自发凝集。将5×10⁶个Ca761 - 86悬浮细胞以不同方式（皮下、足垫内或腹腔内）移植到近交系正常615小鼠体内后，移植成功率达到100%。从未观察到自发缓解，且其转移能力保留。未检测到类胸膜肺炎微生物。Ca761 - 86可能具有实际应用价值。
