• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

系统药理学——剖析血竭改善缺血性中风预后的分子机制

Systems Pharmacology-Dissection of the Molecular Mechanisms of Dragon's Blood in Improving Ischemic Stroke Prognosis.

作者信息

Jiang Meng, Su Xing, Liu Jianling, Zheng Chunli, Li Xiaogang

机构信息

Precision Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Street, Xi'an 710061, China.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Shihezi University, Shihezi 832002, China.

出版信息

Evid Based Complement Alternat Med. 2020 May 17;2020:4858201. doi: 10.1155/2020/4858201. eCollection 2020.

DOI:10.1155/2020/4858201
PMID:32508949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7251463/
Abstract

MATERIALS AND METHODS

(1) Based on system-pharmacology platform, the potential active compounds of DB are screened out according to ADME. (2) The ischemic stroke-related targets are predicted by utilizing these active compounds as probes, mapping the targets to the CTD database to establish a molecular-target-disease network. (3) To analyze the mechanism of DB treatment for the prognosis of ischemic stroke, we used the Metascape and DAVID databases to construct "ischemic stroke pathways". (4) PC12 cells were used to explore the protective effect of loureirin B on oxygen-glucose deprivation/reperfusion (OGD/R) injury, and BV-2 cells were used to determine the anti-inflammation effect of 4',7-dihydroxyflavone.

RESULTS

Finally, we obtained 38 active compounds and 58 stroke-related targets. Network and pathway analysis indicate that DB is effective in the treatment of ischemic stroke by enhancing cell survival and inhibiting inflammatory and antiplatelet activation. In experiments, the main component loureirin B promoted the expression of HO-1 and Bcl-2 via positive regulation of PI3K/AKT/CREB and Nrf2 signaling pathways in PC12 cells against OGD/R damage. And the anti-inflammatory activity of 4',7-dihydroxyflavone was related to the inhibition of COX-2, TNF-, and IL-6 in LPS-induced BV-2 cells.

CONCLUSIONS

In our study, the results illustrated that DB in improving ischemic stroke prognosis may involve enhancing cell survival and antioxidant, anti-inflammation, and antiplatelet activities.

摘要

材料与方法

（1）基于系统药理学平台，根据药物代谢动力学（ADME）筛选出丹参的潜在活性化合物。（2）以这些活性化合物为探针预测缺血性中风相关靶点，将靶点映射到CTD数据库以建立分子靶点-疾病网络。（3）为分析丹参治疗缺血性中风预后的机制，我们使用Metascape和DAVID数据库构建“缺血性中风通路”。（4）用PC12细胞探讨丹酚酸B对氧糖剥夺/再灌注（OGD/R）损伤的保护作用，用BV-2细胞确定4',7-二羟基黄酮的抗炎作用。

结果

最终，我们获得了38种活性化合物和58个中风相关靶点。网络和通路分析表明，丹参通过增强细胞存活、抑制炎症和抗血小板激活有效治疗缺血性中风。在实验中，主要成分丹酚酸B通过PI3K/AKT/CREB和Nrf2信号通路的正向调节促进PC12细胞中HO-1和Bcl-2的表达，以抵抗OGD/R损伤。4',7-二羟基黄酮的抗炎活性与抑制脂多糖诱导的BV-2细胞中COX-2、TNF-α和IL-6有关。

结论

在我们的研究中，结果表明丹参改善缺血性中风预后可能涉及增强细胞存活以及抗氧化、抗炎和抗血小板活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/6139008e2705/ECAM2020-4858201.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/159cbfed84bf/ECAM2020-4858201.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/1db6448ac9c0/ECAM2020-4858201.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/78d4fe11e1be/ECAM2020-4858201.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/43597fc90511/ECAM2020-4858201.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/e0176f89c43f/ECAM2020-4858201.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/d629eb688f4e/ECAM2020-4858201.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/b0e7c63e0a17/ECAM2020-4858201.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/6139008e2705/ECAM2020-4858201.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/159cbfed84bf/ECAM2020-4858201.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/1db6448ac9c0/ECAM2020-4858201.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/78d4fe11e1be/ECAM2020-4858201.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/43597fc90511/ECAM2020-4858201.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/e0176f89c43f/ECAM2020-4858201.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/d629eb688f4e/ECAM2020-4858201.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/b0e7c63e0a17/ECAM2020-4858201.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2a/7251463/6139008e2705/ECAM2020-4858201.008.jpg

相似文献

1
Systems Pharmacology-Dissection of the Molecular Mechanisms of Dragon's Blood in Improving Ischemic Stroke Prognosis.系统药理学——剖析血竭改善缺血性中风预后的分子机制
Evid Based Complement Alternat Med. 2020 May 17;2020:4858201. doi: 10.1155/2020/4858201. eCollection 2020.
2
Senkyunolide H protects PC12 cells from OGD/R-induced injury via cAMP-PI3K/AKT signaling pathway.松柏醇内酯 H 通过 cAMP-PI3K/AKT 信号通路保护 PC12 细胞免受 OGD/R 损伤。
J Ethnopharmacol. 2022 Jan 10;282:114659. doi: 10.1016/j.jep.2021.114659. Epub 2021 Sep 17.
3
Protective effects of the knockdown of lncRNA AK139328 against oxygen glucose deprivation/reoxygenation-induced injury in PC12 cells.lncRNA AK139328 敲低对氧葡萄糖剥夺/复氧诱导的 PC12 细胞损伤的保护作用。
Mol Med Rep. 2021 Sep;24(3). doi: 10.3892/mmr.2021.12260. Epub 2021 Jul 2.
4
Theaflavic acid from black tea protects PC12 cells against ROS-mediated mitochondrial apoptosis induced by OGD/R via activating Nrf2/ARE signaling pathway.红茶中的茶黄素酸通过激活 Nrf2/ARE 信号通路来保护 PC12 细胞免受 OGD/R 诱导的 ROS 介导的线粒体凋亡。
J Nat Med. 2020 Jan;74(1):238-246. doi: 10.1007/s11418-019-01333-4. Epub 2019 Jun 21.
5
Medicarpin Protects Cerebral Microvascular Endothelial Cells Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury via the PI3K/Akt/FoxO Pathway: A Study of Network Pharmacology Analysis and Experimental Validation.豆荚皮素通过PI3K/Akt/FoxO通路保护脑微血管内皮细胞免受氧糖剥夺/复氧诱导的损伤：网络药理学分析与实验验证研究
Neurochem Res. 2022 Feb;47(2):347-357. doi: 10.1007/s11064-021-03449-0. Epub 2021 Sep 14.
6
Germacrone protects against oxygen-glucose deprivation/reperfusion injury by inhibiting autophagy processes in PC12 cells.倍半萜烯通过抑制 PC12 细胞自噬过程来防止氧葡萄糖剥夺/再灌注损伤。
BMC Complement Med Ther. 2020 Mar 7;20(1):77. doi: 10.1186/s12906-020-2865-1.
7
Comprehensive evaluation of Dragon's Blood in combination with borneol in ameliorating ischemic/reperfusion brain injury using RNA sequencing, metabolomics, and 16S rRNA sequencing.运用RNA测序、代谢组学和16S rRNA测序对血竭联合冰片改善缺血/再灌注脑损伤进行综合评价。
Front Pharmacol. 2024 May 9;15:1372449. doi: 10.3389/fphar.2024.1372449. eCollection 2024.
8
Diterpene ginkgolides protect against cerebral ischemia/reperfusion damage in rats by activating Nrf2 and CREB through PI3K/Akt signaling.二萜白果内酯通过激活 PI3K/Akt 信号通路激活 Nrf2 和 CREB 来保护大鼠免受脑缺血/再灌注损伤。
Acta Pharmacol Sin. 2018 Aug;39(8):1259-1272. doi: 10.1038/aps.2017.149. Epub 2018 Mar 15.
9
Knockdown of IL-32 protects PC12 cells against oxygen-glucose deprivation/reoxygenation-induced injury via activation of Nrf2/NF-κB pathway.敲低 IL-32 通过激活 Nrf2/NF-κB 通路保护 PC12 细胞免受氧葡萄糖剥夺/复氧诱导的损伤。
Metab Brain Dis. 2020 Feb;35(2):363-371. doi: 10.1007/s11011-019-00530-0. Epub 2020 Jan 8.
10
Loureirin B: An Effective Component in Dragon's Blood Modulating Sodium Currents in TG Neurons.龙血竭中的有效成分龙血素B对三叉神经节神经元钠电流的调制作用
Conf Proc IEEE Eng Med Biol Soc. 2005;2005:4962-5. doi: 10.1109/IEMBS.2005.1615588.

引用本文的文献

1
Enhancing antioxidant activity of corn bract and silk juices through biotransformation of polyphenols by TJ199 fermentation.通过TJ199发酵对多酚进行生物转化来增强玉米苞叶和玉米须汁液的抗氧化活性。
Food Chem X. 2025 May 26;28:102586. doi: 10.1016/j.fochx.2025.102586. eCollection 2025 May.
2
Dragon's Blood Modulates Disulfidptosis-Related Genes to Alleviate Ischemic Brain Injury in Mice.龙血调节与二硫化物化相关的基因以减轻小鼠缺血性脑损伤。
Neurochem Res. 2025 Jun 6;50(3):185. doi: 10.1007/s11064-025-04428-5.
3
Metabolomics and quantitative analysis to determine differences in the geographical origins and species of Chinese dragon's blood.

本文引用的文献

1
Systems pharmacology uncover the mechanism of anti-non-small cell lung cancer for Hedyotis diffusa Willd.系统药理学揭示白花蛇舌草抗非小细胞肺癌的作用机制。
Biomed Pharmacother. 2019 Jan;109:969-984. doi: 10.1016/j.biopha.2018.10.162. Epub 2018 Nov 5.
2
Prevention of stroke: a global perspective.预防中风：全球视角。
Lancet. 2018 Oct 6;392(10154):1269-1278. doi: 10.1016/S0140-6736(18)31269-8.
3
Molecular Communication of a Dying Neuron in Stroke.脑卒中死亡神经元的分子通讯
代谢组学与定量分析以确定中国血竭地理来源和品种的差异。
Front Plant Sci. 2024 Sep 18;15:1427731. doi: 10.3389/fpls.2024.1427731. eCollection 2024.
4
Loureirin B inhibits Cervical Cancer Development by Blocking PI3K/AKT Signaling Pathway: Network Pharmacology Analysis and Experimental Validation.龙血素B通过阻断PI3K/AKT信号通路抑制宫颈癌发展：网络药理学分析与实验验证
Appl Biochem Biotechnol. 2024 Dec;196(12):8587-8604. doi: 10.1007/s12010-024-04975-7. Epub 2024 Jun 18.
5
Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by Simulated Microgravity.龙血竭通过调控 Rac1-WAVE2-Arp2/3 信号通路保护模拟失重大鼠肠上皮屏障功能障碍
Int J Mol Sci. 2021 Mar 8;22(5):2722. doi: 10.3390/ijms22052722.
Int J Mol Sci. 2018 Sep 19;19(9):2834. doi: 10.3390/ijms19092834.
4
Trends in Deep Vein Thrombosis Prophylaxis and Deep Vein Thrombosis Rates After Total Hip and Knee Arthroplasty.全髋关节和膝关节置换术后深静脉血栓形成预防和深静脉血栓形成发生率的趋势。
J Am Acad Orthop Surg. 2018 Oct 1;26(19):698-705. doi: 10.5435/JAAOS-D-17-00235.
5
Method development and application for multi-component quantification in rats after oral administration of Longxuetongluo Capsule by UHPLC-MS/MS.建立 UHPLC-MS/MS 法测定大鼠灌胃龙血通络胶囊后多成分的含量。
J Pharm Biomed Anal. 2018 Jul 15;156:252-262. doi: 10.1016/j.jpba.2018.04.030. Epub 2018 Apr 22.
6
Diterpene ginkgolides protect against cerebral ischemia/reperfusion damage in rats by activating Nrf2 and CREB through PI3K/Akt signaling.二萜白果内酯通过激活 PI3K/Akt 信号通路激活 Nrf2 和 CREB 来保护大鼠免受脑缺血/再灌注损伤。
Acta Pharmacol Sin. 2018 Aug;39(8):1259-1272. doi: 10.1038/aps.2017.149. Epub 2018 Mar 15.
7
Current and future antiplatelet therapies: emphasis on preserving haemostasis.当前和未来的抗血小板治疗：强调保持止血功能。
Nat Rev Cardiol. 2018 Mar;15(3):181-191. doi: 10.1038/nrcardio.2017.206. Epub 2018 Jan 3.
8
In silico-based screen synergistic drug combinations from herb medicines: a case using Cistanche tubulosa.基于计算机的草药协同药物组合筛选：以肉苁蓉为例。
Sci Rep. 2017 Nov 27;7(1):16364. doi: 10.1038/s41598-017-16571-3.
9
Anti-atherosclerotic effect of Longxuetongluo Capsule in high cholesterol diet induced atherosclerosis model rats.龙血通络胶囊对高胆固醇饮食诱导动脉粥样硬化模型大鼠的抗动脉粥样硬化作用。
Biomed Pharmacother. 2018 Jan;97:793-801. doi: 10.1016/j.biopha.2017.08.141. Epub 2017 Nov 6.
10
Characterization and quantitative analysis of phenolic derivatives in Longxuetongluo Capsule by HPLC-DAD-IT-TOF-MS.高效液相色谱-二极管阵列检测-离子阱-飞行时间质谱法对龙血通络胶囊中酚类衍生物的表征及定量分析
J Pharm Biomed Anal. 2017 Oct 25;145:462-472. doi: 10.1016/j.jpba.2017.07.012. Epub 2017 Jul 14.