Duan Renjie, Wang Yue, Lin Aiqin, Lian Likai, Cao Huiru, Gu Wenjie, Li Tiechen, Sun Qing
School of Preclinical Medicine, Wannan Medical College Wuhu, Anhui, China.
Department of Gynaecology and Obstetrics, Yijishan Hospital of Wannan Medical College Wuhu, Anhui, China.
Int J Clin Exp Pathol. 2020 May 1;13(5):1024-1029. eCollection 2020.
To investigate the expression and clinical significance of nucleoside diphosphate kinase A (nm23-H1), p53, and integrin β1 in endometriosis, normal and ectopic endometrial tissues were collected and the levels of nm23-H1, p53, and integrin β1 proteins were detected by western blotting. We also measured the mRNA expression of nm23-H1, p53, and integrin β1 in endometrial epithelial cells by droplet digital PCR, based on endometrial tissues using laser capture microdissection. Moreover, primary stromal cells from normal and ectopic endometrial tissues were also cultured and treated with different concentrations of estrogen. We assessed the mRNA levels of nm23-H1, p53, and integrin β1 by quantitative PCR. Compared with normal endometrial tissue, the levels of nm23-H1 and p53 proteins were significantly downregulated in ectopic endometrial tissues, while integrin β1 protein was upregulated. The same expression trend in the mRNA levels of nm23-H1, p53, and integrin β1 was also observed in both endometrial epithelial cells and stromal cells. In addition, with increasing estrogen concentration, nm23-H1 and p53 mRNA levels gradually decreased, while integrin β1 mRNA expression increased. Nm23-H1 and p53 may inhibit the progression of endometriosis, while integrin β1 has a promoting effect, and estrogen is involved in this process.
为研究核苷二磷酸激酶A（nm23-H1）、p53和整合素β1在子宫内膜异位症中的表达及临床意义，收集正常及异位子宫内膜组织，采用蛋白质免疫印迹法检测nm23-H1、p53和整合素β1蛋白水平。基于使用激光捕获显微切割技术获取的子宫内膜组织，我们还通过液滴数字PCR测定了子宫内膜上皮细胞中nm23-H1、p53和整合素β1的mRNA表达。此外，还培养了来自正常及异位子宫内膜组织的原代基质细胞，并用不同浓度的雌激素进行处理。我们通过定量PCR评估nm23-H1、p53和整合素β1的mRNA水平。与正常子宫内膜组织相比，异位子宫内膜组织中nm23-H1和p53蛋白水平显著下调，而整合素β1蛋白上调。在子宫内膜上皮细胞和基质细胞中也观察到nm23-H1、p53和整合素β1的mRNA水平呈现相同的表达趋势。此外，随着雌激素浓度增加，nm23-H1和p53的mRNA水平逐渐降低，而整合素β1的mRNA表达增加。Nm23-H1和p53可能抑制子宫内膜异位症的进展，而整合素β1具有促进作用，且雌激素参与了这一过程。
