Steinkellner Hannes, Beribisky Alexander V, Mausberg Philip, Christodoulou John, Scheiber-Mojdehkar Barbara, Huber Anna, Sarne Victoria, Laccone Franco
Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV);
Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV).
J Vis Exp. 2020 May 22(159). doi: 10.3791/61054.
The ECLIA is a versatile method which is able to quantify endogenous and recombinant protein amounts in a 96-well format. To demonstrate ECLIA efficiency, this assay was used to analyze intrinsic levels of MeCP2 in mouse brain tissue and the uptake of TAT-MeCP2 in human dermal fibroblasts. The MeCP2-ECLIA produces highly accurate and reproducible measurements with low intra- and inter-assay error. In summary, we developed a quantitative method for the evaluation of MeCP2 protein variants that can be utilized in high-throughput screens.
电化学发光免疫分析（ECLIA）是一种通用方法，能够以96孔板形式对内源性和重组蛋白量进行定量。为证明ECLIA的效率，该检测方法用于分析小鼠脑组织中MeCP2的内在水平以及人真皮成纤维细胞中TAT-MeCP2的摄取。MeCP2-ECLIA检测具有高度准确和可重复的测量结果，且批内和批间误差低。总之，我们开发了一种用于评估MeCP2蛋白变体的定量方法，可用于高通量筛选。
