Benslimane Fatiha M, Zakaria Zain Z, Shurbaji Samar, Abdelrasool Mahmoud Khatib Ali, Al-Badr Mashael Ali H I, Al Absi Enas Said Khalil, Yalcin Huseyin C
Biomedical Research Center, Qatar University, Doha, Qatar.
Biomedical Research Center, Qatar University, Doha, Qatar; Department of Biological and Environmental Sciences, College of Arts and Science, Qatar University, Doha, Qatar.
Micron. 2020 Sep;136:102876. doi: 10.1016/j.micron.2020.102876. Epub 2020 May 19.
In the last few decades, zebrafish (Danio rerio) were introduced as a model organism to investigate human diseases including cardiovascular and neuronal disorders. In most zebrafish investigations, cardiac function and blood flow hemodynamics need to be assessed to study the effects of the interference on the cardiovascular system. For heart function assessment, most important parameters include heart rate, cardiac output, ejection fraction, fractional area change, and fractional shortening.
A 10 s high-speed video of beating heart and flowing blood within major vessels of zebrafish that are less than 5 days post fertilization (dpf) were recorded via a stereo microscope equipped with a high speed camera. The videos were analyzed using MicroZebraLab and image J software for the assessment of cardiac function.
Using the technique described here, we were able to simply yet effectively assess cardiac function and blood flow dynamics of normal zebrafish embryos. We believe that the practical method presented here will help cardiac researchers using the zebrafish as a model to examine cardiac function by using tools that could be available in their laboratory.
在过去几十年中，斑马鱼（Danio rerio）被引入作为一种模式生物，用于研究包括心血管和神经疾病在内的人类疾病。在大多数斑马鱼研究中，需要评估心脏功能和血流动力学，以研究干扰对心血管系统的影响。对于心脏功能评估，最重要的参数包括心率、心输出量、射血分数、面积变化分数和缩短分数。
通过配备高速相机的立体显微镜，记录受精后不到5天（dpf）的斑马鱼心脏跳动和主要血管内血液流动的10秒高速视频。使用MicroZebraLab和图像J软件对视频进行分析，以评估心脏功能。
使用此处描述的技术，我们能够简单而有效地评估正常斑马鱼胚胎的心脏功能和血流动力学。我们相信，此处介绍的实用方法将帮助以斑马鱼为模型的心脏研究人员，利用其实验室中可用的工具来检查心脏功能。
