Remold H G, Mednis A D
J Immunol. 1977 Jun;118(6):2015-9.
Migration inhibitory factor (MIF), produced by stimulation of guinea pig lymph node cells with concanavalin A, was fractionated by Sephadex G-100 gel filtration, sucrose density gradient electrophoresis, and isoelectrofocussing. Two distinct species were identified and separated. One, pH 3-MIF, has an isoelectric point of 3.0 to 4.5 and elutes from Sephadex G-75 columns with molecules having an apparent m.w. of 65,000 (Kd of 0.05 to 0.12). The other, pH 5-MIF, has an isoelectric point of 5.0 to 5.5 and elutes with molecules having an apparent m.w. between 25,000 and 43,000 (Kd of 0.15 to 0.23).
用伴刀豆球蛋白A刺激豚鼠淋巴结细胞产生的迁移抑制因子（MIF），通过Sephadex G - 100凝胶过滤、蔗糖密度梯度电泳和等电聚焦进行分级分离。鉴定并分离出两种不同的组分。一种是pH 3 - MIF，其等电点为3.0至4.5，从Sephadex G - 75柱上洗脱时，其表观分子量为65,000（分配系数Kd为0.05至0.12）。另一种是pH 5 - MIF，其等电点为5.0至5.5，洗脱时的表观分子量在25,000至43,000之间（分配系数Kd为0.15至0.23）。
