Kuhlmann L, Steinsträsser A
Hoechst Aktiengesellschaft, Frankfurt/Main, F.R.G.
Int J Rad Appl Instrum B. 1988;15(6):617-27. doi: 10.1016/0883-2897(88)90052-9.
When used for immunoscintigraphic detection of malignant tumours, monoclonal antibodies are frequently labelled with indium-111. This study investigated whether antibody properties are affected by DTPA, the chelating agent with which the antibody has to be bound before labelling with indium-111. Chromatography, cell binding assays and animal studies indicated marked changes in the chemical, immunological and biological properties of the F(ab')2 antibody fragment 431/31 at DTPA:F(ab')2 ratios greater than 1. It is concluded that, in order to avoid lasting effects on the normal biokinetic behaviour of this antibody, the DTPA:F(ab')2 ratio for the antibody should not exceed 1.
当用于恶性肿瘤的免疫闪烁检测时，单克隆抗体常被用铟 - 111标记。本研究调查了抗体性质是否会受到二乙三胺五乙酸（DTPA）的影响，DTPA是一种螯合剂，抗体在用铟 - 111标记之前必须与之结合。色谱分析、细胞结合试验和动物研究表明，当DTPA与F(ab')2抗体片段431/31的比例大于1时，F(ab')2抗体片段的化学、免疫和生物学性质会发生显著变化。得出的结论是，为避免对该抗体的正常生物动力学行为产生持久影响，抗体的DTPA与F(ab')2比例不应超过1。
