基于肽核酸（PNA）导向的壳交联（SCK）纳米颗粒缀合物的溶液相和表面组装

PNA-directed solution- and surface-assembly of shell crosslinked (SCK) nanoparticle conjugates.

作者信息

Turner Jeffrey L, Becker Matthew L, Li Xiaoxu, Taylor John-Stephen A, Wooley Karen L

机构信息

Center for Materials Innovation and Department of Chemistry, Washington University, One Brookings Drive, CB 1134, St. Louis, Missouri63130-4899, USA.

出版信息

Soft Matter. 2005 May 27;1(1):69-78. doi: 10.1039/b417653g.

DOI:10.1039/b417653g

PMID:32521833

Abstract

The conjugation of complementary peptide nucleic acid (PNA) sequences to well defined shell crosslinked (SCK) nanoparticles is reported as a mechanism by which to direct their self assembly into higher order structures selective and tunable binding interactions. Base-pairing-driven aggregation of the SCK's occurred for mixtures of SCK's that presented complementary sequences in aqueous sodium chloride solutions and upon mica substrates. The assembly processes were monitored by dynamic light scattering and atomic force microscopy as a function of salt concentration, and by UV-vis spectroscopy as a function of salt concentration and temperature. Moreover, the stoichiometries of the PNA sequences conjugated per SCK nanoparticle and the stoichiometric ratios in the production of mixtures of SCK's bearing complementary PNA sequences were each altered to tune the hierarchical assemblies.

摘要

据报道，将互补肽核酸（PNA）序列与定义明确的壳交联（SCK）纳米颗粒进行共轭，是一种引导它们自组装成具有选择性和可调谐结合相互作用的高阶结构的机制。在氯化钠水溶液和云母基底上，呈现互补序列的SCK混合物发生了由碱基配对驱动的聚集。通过动态光散射和原子力显微镜监测组装过程与盐浓度的关系，通过紫外可见光谱监测组装过程与盐浓度和温度的关系。此外，改变每个SCK纳米颗粒共轭的PNA序列的化学计量以及生产带有互补PNA序列的SCK混合物时的化学计量比，以调节分级组装。