Institute of Oncology and Radiology of Serbia, Department of Experimental Oncology, Pasterova 14,11000 Belgrade, Serbia.
J BUON. 2020 Mar-Apr;25(2):1219-1229.
Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco.
Fractions were made from dichloromethane:methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method.
Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity.
The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
海洋大型藻类是具有重要生物价值化合物的重要来源。本研究的主要目的是研究来自摩洛哥海岸线的石莼（Fucus spiralis）二氯甲烷-甲醇提取物和三个馏分的体外抗癌特性和化学成分。
馏分是由二氯甲烷：甲醇（1：1）的石莼提取物制成的：石油醚、乙酸乙酯和正丁醇。提取物和馏分通过 MTT 测定法对人宫颈腺癌（HeLa）、结肠直肠腺癌（LS-174T）、肺癌（A549）和正常人肺成纤维细胞（MRC-5）进行体外细胞毒性筛选。使用流式细胞术评估 HeLa 细胞的细胞周期分布。使用吖啶橙（AO）-溴化乙锭（EB）染色在荧光显微镜下评估 HeLa 细胞的形态变化。使用划痕和管形成测定法对人内皮衍生的永久 EA.hy926 细胞系研究抗迁移和抗血管生成特性。使用抗-α-葡萄糖苷酶测定法测试抗糖尿病活性。使用微量稀释法测试抗菌效果。
富含脂肪酸的石莼石油醚馏分对 HeLa 细胞表现出最高的细胞毒性。乙酸乙酯和石油醚馏分诱导 HeLa 细胞在 sub-G1 和 G2/M 期积累最多。提取物和馏分在荧光显微镜下对 HeLa 细胞表现出促凋亡作用。它们在体外显示出抗迁移和抗血管生成作用。α-葡萄糖苷酶抑制活性的 IC50 值强于标准阿卡波糖。正丁醇馏分表现出最强的抗菌和抗真菌活性。
对从石莼中获得的提取物和馏分的各种生物活性的研究表明，这种可食用的大型藻类具有有希望的抗癌和药理学潜力。
