Albadawi Dina A, Mothana Ramzi A, Khaled Jamal M, Ashour Abdelkhader E, Kumar Ashok, Ahmad Sheikh F, Al-Said Mansour S, Al-Rehaily Adnan J, Almusayeib Nawal M
a Department of Natural Products and Alternative Medicine, College of Pharmacy , King Abdulaziz University , Jeddah , Saudi Arabia.
b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.
Pharm Biol. 2017 Dec;55(1):1759-1766. doi: 10.1080/13880209.2017.1322617.
Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage.
This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents.
The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay.
The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7).
Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action.
树脂臭黄荆（Premna resinosa (Hochst.) Schauer，唇形科）在许多地方被用于治疗支气管炎、呼吸道疾病和胸廓抽搐。
本研究评估树脂臭黄荆的抗癌、抗菌和抗氧化活性，并分离出一些起作用的成分。
测试了树脂臭黄荆地上部分的甲醇提取物及其馏分（正己烷、二氯甲烷、乙酸乙酯和正丁醇）。采用微量稀释法对三种革兰氏阳性菌和四种革兰氏阴性菌测试抗菌活性。测试浓度范围为4000至7.8μg/mL，孵育24小时后测定最低抑菌浓度（MIC）值。使用MTT法对三种人类癌细胞系（道氏细胞（Daoy）、肝癌细胞系HepG2和黑色素瘤细胞系SK-MEL28）评估抗癌活性。通过DPPH清除法和β-胡萝卜素-亚油酸测定法研究抗氧化活性。
正己烷馏分对金黄色葡萄球菌、粪肠球菌和弗氏志贺氏菌表现出最大的抗菌活性（MIC为10μg/mL）。正己烷馏分对道氏细胞、肝癌细胞系HepG2和黑色素瘤细胞系SK-MEL28细胞系诱导出最大的细胞毒性活性，IC值分别为9.0、8.5和13.2。此外，二氯甲烷和乙酸乙酯馏分显示出最高的抗氧化潜力。生物活性导向的分级分离首次导致分离和鉴定出七种化合物，即槲皮素（1）、3-甲氧基槲皮素（2）、山奈酚（3）、3-甲氧基山奈酚（4）、杨梅素3,7,3'-三甲醚（5）、羽扇豆醇（6）和豆甾醇（7）。
我们的结果表明树脂臭黄荆是抗菌和细胞毒性化合物的一个来源。然而，需要进一步的工作来分离其他活性成分并确定其作用机制。
