Gaspareto Taís Carla, Scapini Thamarys, Venturin Bruno, Tápparo Deisi Cristina, Camargo Aline Frumi, Luccio Marco Di, Valerio Alexsandra, Favretto Rafael, Antes Fabiane Goldschmidt, Steinmetz Ricardo Luís Radis, Treichel Helen, Kunz Airton
Laboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil.
Western Paraná State University, Cascavel, Brazil.
Front Bioeng Biotechnol. 2020 May 25;8:477. doi: 10.3389/fbioe.2020.00477. eCollection 2020.
We studied different pre-treatments of poultry litter aiming to add economic value to this residue. Strategies were applied to extract ammonium nitrogen with the aim of allowing its further use as fertilizer, and to promote the hydrolysis and solubilization of lignocellulosic components with the aim of facilitating its subsequent conversion to biogas. Ammonia extraction was performed by solubilization in water in a one-step process and by successive extraction steps (3 times 60 min). Successive extractions presented greater removal of total ammonia nitrogen than did one-step extraction, solubilizing about 36% of the ammonia in water. In parallel pre-treatment using ultrasound was performed to increase carbon bioavailability for anaerobic digestion. Using this tool, 24.7 g kg of total organic carbon and 13.0 g kg of total reducing sugars were solubilized, employing 10% dry mass sample amount, 100% amplitude ultrasound at frequency of 20 kHz amplitude and 2.5 min of treatment (energy input of 299 ± 7 kJ L; 3,822 ± 95 kJ kg). Anaerobic digestion of ultrassound pre-treated biomass was evaluated using a biological biogas production assay, and an increase of 10% of biogas production was obtained compared to untreated samples (147.9 and 163.0 mL g for crude and pre-treated PL, respectively). The findings suggest that these are environmentally friendly and sustainable strategies to add economic value to poultry litter, reducing the environmental impacts of improper disposal.
我们研究了家禽粪便的不同预处理方法，旨在为这种残渣增加经济价值。应用了多种策略来提取铵态氮，以便其能进一步用作肥料，并促进木质纤维素成分的水解和溶解，从而便于其后续转化为沼气。氨提取通过一步水溶法和连续提取步骤（3次，每次60分钟）进行。连续提取比一步提取能去除更多的总氨氮，能使约36%的氨溶解于水中。同时，采用超声进行预处理以提高厌氧消化时的碳生物可利用性。使用该工具，以10%的干质量样品量、20 kHz频率、100%振幅的超声处理2.5分钟（能量输入为299±7 kJ/L；3822±95 kJ/kg），可溶解24.7 g/kg的总有机碳和13.0 g/kg的总还原糖。通过生物沼气生产试验评估了超声预处理生物质的厌氧消化情况，与未处理样品相比，沼气产量增加了10%（粗制家禽粪便和预处理家禽粪便分别为147.9和163.0 mL/g）。研究结果表明，这些是为家禽粪便增加经济价值、减少不当处置对环境影响的环保且可持续的策略。
