Volfova Martina, Machovcova Zuzana, Voslarova Eva, Bedanova Iveta, Vecerek Vladimir
Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, 612 42 Brno, Czech Republic.
Animals (Basel). 2020 Jun 10;10(6):1013. doi: 10.3390/ani10061013.
We compared the glucocorticoid concentrations in response to various types of potential stressors present during standard operation of a temporary housing facility between three species, namely, ring-tailed lemurs, collared brown lemurs and white-headed lemurs. The levels of faecal glucocorticoid metabolites (FGMs) were measured non-invasively on a daily basis during a 30-day period. A total of 510 faecal samples were collected. Concentrations of immunoreactive glucocorticoid hormone metabolites were measured in the obtained extracts by using an enzyme immunoassay. The polyclonal antibodies used in this assay were directed against the metabolite 11-oxo-etiocholanolone I. We found all three monitored lemur species to respond to specific potentially stressful situations by increasing ( < 0.05) the FGM levels within one to two days after the event. Although housed in the same room, differences in response to potentially stressful situations were found in white-headed lemurs compared to ring-tailed lemurs. Increased mean levels of the FGMs were found more frequently in white-headed lemurs than in ring-tailed lemurs. The results suggest that this species may be more sensitive to changes in its surroundings. In general, the levels of the FGMs showed a similar pattern during 30 days of monitoring suggesting that all groups of lemurs responded in a similar manner to the same events. However, we recorded the differences in the absolute values of glucocorticoid concentrations between the monitored species likely due to the differences in sex ratios in the groups and presence of lactating females in the ring-tailed lemurs.
我们比较了环尾狐猴、领狐猴和白头狐猴这三个物种在临时居住设施标准运营期间，对各种潜在应激源的糖皮质激素浓度反应。在30天的时间里，每天都对粪便糖皮质激素代谢物（FGMs）水平进行非侵入性测量。总共收集了510份粪便样本。通过酶免疫测定法测量所得提取物中免疫反应性糖皮质激素代谢物的浓度。本测定中使用的多克隆抗体针对代谢物11-氧代-雄甾烷醇酮I。我们发现，所有三种被监测的狐猴物种在事件发生后的一到两天内，都会通过提高（<0.05）FGMs水平来应对特定的潜在应激情况。尽管它们被安置在同一个房间，但与环尾狐猴相比，白头狐猴在应对潜在应激情况时存在差异。白头狐猴中FGMs平均水平升高的情况比环尾狐猴更频繁。结果表明，该物种可能对其周围环境的变化更为敏感。总体而言，在30天的监测期间，FGMs水平呈现出相似的模式，这表明所有狐猴群体对相同事件的反应方式相似。然而，我们记录到被监测物种之间糖皮质激素浓度绝对值存在差异，这可能是由于群体中性别比例的差异以及环尾狐猴群体中存在哺乳期雌性。
