Yaremchuk O, Soroka Yu, Kulitska M, Kuzmak I, Chernyashova V, Namoradze M, Delibashvili D, Posokhova K
1I. Horbachevsky Ternopil National Medical University, Ukraine.
2Tbilisi State Medical University, Georgia.
Georgian Med News. 2020 Apr(301):159-165.
The aim of the research was to investigate the effect of aminoguanidine on the content of autoantibodies in the serum, nitric oxide synthesis (NO), glial fibrillary acidic protein (GFAP) content and indicators of free radical oxidation in the cerebral hemispheres of BALB/c mice with antiphospholipid syndrome (APS) on the 18th day of pregnancy. An increase in the content of autoantibodies to brain proteins (120 kDa, 150 kDa, and >170 kDa) was detected in the serum of BALB/c mice with APS on the 18th day of pregnancy. An increase in the content of GFAP (total), GFAP (49-37 kDa), NO2¯, NO3¯ and prooxidant-antioxidant system imbalance in the cerebral hemispheres of pregnant mice with APS was established. With administration of aminoguanidine into the pregnant mice with APS, a decrease in the content of autoantibodies to brain proteins (120 kDa and 150 kDa) in serum was proved. With the introduction of aminoguanidine, a selective inhibitor of inducible NO synthase on the 18th day of pregnancy the increase in GFAP (49-37 kDa) in the cerebral hemispheres of APS mice was established, and the GFAP (total), NO2¯ and NO3¯ content did not change significantly, relative to the indicators of pregnant animals with APS. With introduction of aminoguanidine in cases of APS on the 18th day of pregnancy lesser manifestations of oxidative stress in the cerebral hemispheres, a decrease in the activity of lipid peroxidation processes, an increase in the activity and content of the antioxidant system components was evidenced. Thus, aminoguanidine has a neuroprotective effect in obstetric antiphospholipid syndrome in the BALB/c mice.
该研究的目的是调查氨基胍对妊娠第18天患有抗磷脂综合征（APS）的BALB/c小鼠血清中自身抗体含量、一氧化氮合成（NO）、胶质纤维酸性蛋白（GFAP）含量以及大脑半球自由基氧化指标的影响。在妊娠第18天患有APS的BALB/c小鼠血清中，检测到针对脑蛋白（120 kDa、150 kDa和>170 kDa）的自身抗体含量增加。已证实患有APS的妊娠小鼠大脑半球中GFAP（总量）、GFAP（49 - 37 kDa）、NO2¯、NO3¯含量增加以及促氧化 - 抗氧化系统失衡。给患有APS的妊娠小鼠施用氨基胍后，血清中针对脑蛋白（120 kDa和150 kDa）的自身抗体含量降低得到证实。在妊娠第18天引入诱导型一氧化氮合酶的选择性抑制剂氨基胍后，已证实APS小鼠大脑半球中GFAP（49 - 37 kDa）增加，并且相对于患有APS的妊娠动物指标，GFAP（总量）、NO2¯和NO3¯含量没有显著变化。在妊娠第18天患有APS的情况下引入氨基胍后，大脑半球氧化应激表现较轻，脂质过氧化过程活性降低，抗氧化系统成分的活性和含量增加得到证实。因此，氨基胍对BALB/c小鼠产科抗磷脂综合征具有神经保护作用。
