Zhao Qi-An, Liu Bo, Zhang Yue, Liu Qin, Zhang Li-Huan, Liu Rui-Fang, Yang Ning
College of Life Sciences, Northwest Normal University, 730070, Lanzhou, China.
Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, Lanzhou 730000, China.
Ying Yong Sheng Tai Xue Bao. 2020 Mar;31(3):959-968. doi: 10.13287/j.1001-9332.202003.035.
We investigated the signal relationship between phospholipase Dα1 (PLDα1) and the gas signal molecule hydrogen sulfide (HS) in Arabidopsis thaliana response to the allelopathy of diterpenoid oridonin. The wild type Arabidopsis Columbia (WT), phospholipase Dα1 (PLDα1) deletion mutant pldα1, D-/L-cysteine desulfyrase synthetic deletion mutant d-cdes and l-cdes seedlings were used as experiment materials, while 60 μmol·L oridonin was applied as treatment concentration. The results showed that oridonin significantly increased HS content, PLD and D-/L-CDes activities, and gene expressions of PLDα1 and D-/L-CDes in WT. Under oridonin treatment, the D-CDes and L-CDes activities of pldɑ1 seedlings were significantly lower than those of WT. Both D-CDes and L-CDes activities increased after exogenous addition of phosphatidic acid (PA) and were higher than those of WT. Oridonin significantly inhibited root growth of four lines, with d-cdes and l-cdes being more sensitive to oridonin. Application of NaHS promoted root growth and endogenous HS production of four lines under oridonin treatment, while application of PA increased root growth and endogenous HS production in WT, pldɑ1 and l-cdes, but had no effect in d-cdes. These results indicated that PLDα1 and HS played a vital role in driving the response of Arabidopsis to oridonin, and that PLDα1/PA was located at the upstream of D-CDes to participate the regulation of the HS production and root growth.
我们研究了拟南芥在应对二萜类冬凌草甲素化感作用时，磷脂酶Dα1（PLDα1）与气体信号分子硫化氢（HS）之间的信号关系。以野生型拟南芥哥伦比亚（WT）、磷脂酶Dα1（PLDα1）缺失突变体pldα1、D-/L-半胱氨酸脱硫酶合成缺失突变体d-cdes和l-cdes幼苗作为实验材料，处理浓度为60 μmol·L冬凌草甲素。结果表明，冬凌草甲素显著增加了WT中HS含量、PLD和D-/L-CDes活性以及PLDα1和D-/L-CDes的基因表达。在冬凌草甲素处理下，pldɑ1幼苗的D-CDes和L-CDes活性显著低于WT。外源添加磷脂酸（PA）后，D-CDes和L-CDes活性均增加且高于WT。冬凌草甲素显著抑制了四个株系的根生长，d-cdes和l-cdes对冬凌草甲素更敏感。在冬凌草甲素处理下，施用NaHS促进了四个株系的根生长和内源性HS产生，而施用PA增加了WT、pldɑ1和l-cdes的根生长和内源性HS产生，但对d-cdes没有影响。这些结果表明，PLDα1和HS在驱动拟南芥对冬凌草甲素的反应中起重要作用，并且PLDα1/PA位于D-CDes的上游，参与HS产生和根生长的调节。
